Mouse Hormone-sensitive lipase(LIPE) ELISA kit

Instructions
Code CSB-EL012975MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lipase, hormone-sensitive
Alternative Names Lipe ELISA Kit; Hormone-sensitive lipase ELISA Kit; HSL ELISA Kit; EC 3.1.1.79 ELISA Kit
Abbreviation LIPE
Uniprot No. P54310
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 U/mL-10 U/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 84-93
1:2 Average % 107
Range % 103-110
1:4 Average % 85
Range % 81-89
1:8 Average % 89
Range % 84-93
Recovery
The recovery of rat IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 107 103-110
EDTA plasma (n=4) 86 83-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.100 2.168 2.134 1.995
800 1.654 1.604 1.629 1.490
400 1.181 1.204 1.193 1.054
200 0.765 0.778 0.772 0.633
100 0.517 0.502 0.510 0.371
50 0.346 0.351 0.349 0.210
25 0.228 0.231 0.230 0.091
0 0.140 0.138 0.139  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Gene References into Functions
  1. HSL-deficient mice revealed a complex interorgan interaction between adipose tissue and liver: the role of HSL in the liver is minimal but adipose tissue deficiency of HSL can cause age-dependent hepatic steatosis PMID: 29232702
  2. Cardiac PLIN2 plays an important pathophysiological role in the development of dynamic steatosis and that the latter was prevented by upregulation of intracellular lipases, including Hormone-sensitive Lipase. PMID: 28851734
  3. Enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles which is HSL/ATGL-independent. PMID: 28488768
  4. HSL ablation alters the density of lipid-raft microdomains in mouse testis. HSL-/- lipid rafts had less desmosterol and T-MAS; non-raft had more cholesterol.Monounsaturated acyl phospholipids were higher in raft than in non-raft fractions.HSL ablation reduced PUFA-phospholipids in both raft and non-raft fractions. PMID: 27355565
  5. ase. Similar changes of GPNMB and G0S2 expression were present in a human liposarcoma database. These results show that a previously-unknown, fully penetrant epistatic interaction between Pnpla2 and Lipe can cause liposarcoma in mice. DAKO mice provide a promising model for studying early premalignant changes that lead to late-onset malignant disease. PMID: 28459858
  6. Activities of adipose triglyceride lipase (ATGL), hormone sensitive lipolitic enzyme (HSL) and monoacylglycerol lipase (MGL) were significantly higher (51 %, 38 %, 49 %) in the DE group than the HF group (p < 0.05). MGL, there were no differences between the CO group, HF group, and DC group, with the DE group (70 %) being significantly higher (p < 0.05). PMID: 27596982
  7. Results clearly indicate that SF-1 is involved in the regulation of LIPE expression after activation of protein kinase A in adrenocortical cells. PMID: 26122391
  8. A role for HSL in kidney lipolysis:fasting up-regulates HSL levels and phosphorylation in mouse kidney. PMID: 25879679
  9. Data suggest that cardiotrophin-1 up-regulates lipolysis in white adipocytes via 1) induction of perilipin, 2) activation of HSL (via phosphorylation by PKA), and 3) inactivation of adipose triglyceride lipase (via up-regulation of inhibitor G0S2). PMID: 25351614
  10. QRFP-43 attenuates lipolysis by preventing the formation of an active complex between perilipin A, caveolin-1, the catalytic subunit of protein kinase and hormone-sensitive lipase on lipid droplets. PMID: 25677823
  11. PRIP promotes the translocation of phosphatases to lipid droplets to trigger the dephosphorylation of HSL and perilipin A, thus reducing PKA-mediated lipolysis. PMID: 24945349
  12. Whereas the catalytic function of HSL is necessary for spermatogenesis in mice, the presence of the N-terminal testis-specific fragment is not essential. PMID: 24797631
  13. Changes in fatty acid metabolism observed in testes from HSL-knockout male mice may underlie the infertility observed in these animals. PMID: 23369366
  14. HSL acts to drive cAMP/PKA-mediated regulation of StAR expression and steroidogenesis in mouse Leydig cells. PMID: 23362264
  15. High-fat diet strongly reduced HSL phosphorylation at Ser660 in mouse skeletal muscle. PMID: 23471217
  16. analysis ofsubstrate and stereo/regioselectivity of adipose triglyceride lipase, hormone-sensitive lipase, and diacylglycerol-O-acyltransferases PMID: 23066022
  17. Loss of hormone sensitive lipase is associated with Cholesteryl ester accumulation and accelerated cholesterol absorption in intestine. PMID: 22842588
  18. Resveratrol increased adipose triglyceride lipase gene and protein expressions, an effect that was not observed for hormone-sensitive lipase in mice 3T3-L1 adipocytes. PMID: 21543206
  19. the dissociation between cardiac steatosis and functional sequelae observed in HSL knockout mice suggests that excess fatty acid influx, rather than steatosis appears to play an important role in the pathogenesis of cardiac lipotoxicity PMID: 19706782
  20. functional roles of adipose triglyceride lipase and hormone-sensitive lipase in TNF-alpha-induced lipolysis PMID: 21969372
  21. Study illustrates the importance of HSL for normal lipid metabolism in response to a high fat diet. PMID: 21738729
  22. The absence of HSL directs cells within the bone marrow toward osteoblast differentiation and favors the maintenance of bone density with aging. PMID: 21566206
  23. findings show that the inhibition of lipolysis through genetic ablation of adipose triglyceride lipase or hormone-sensitive lipase ameliorates certain features of cancer-associated cachexia PMID: 21680814
  24. HSL modulates adipose metabolism is by providing intrinsic ligands or pro-ligands for PPARgamma. PMID: 20950707
  25. Fatty acid synthase and hormone-sensitive lipase expression in liver are involved in zinc-alpha2-glycoprotein-induced body fat loss in obese mice. PMID: 21180279
  26. Elevated B56alpha/PP2A inhibits HSL and lipolysis in white adipose tissue of DIO mice. PMID: 20534721
  27. The interaction of hormone-sensitive lipase with vimentin, and its hormonal dependence, was confirmed by coimmunoprecipitation. PMID: 20143880
  28. Data suggest that upregulation of adipose triglyceride lipase and suppression of hormone-sensitive lipase and AMP kinase signaling mediate high-fat diet-induced alterations in lipolysis and lipid utilization in adipocytes. PMID: 20107043
  29. Data demonstrate that HSL can interact with an N-terminal region located between amino acids 141 and 200 of Plin A as well as with a C-terminal region located between amino acids 406 and 480. PMID: 19515989
  30. Results identify a link between hormone-sensitive lipase, and suggest that disturbances in polyamine metabolism may affect insulin sensitivity. PMID: 19785415
  31. perilipin expression and phosphorylation state are critical regulators of lipid storage and hydrolysis in ACS1/FATP1 cells PMID: 11751901
  32. deficiency changes the plasma lipid profile by affecting the tissue-specific expression pattern of lipoprotein lipase in adipose tissue and muscle PMID: 11809748
  33. Hormone-sensitive lipase-deficient mice accumulate lipid droplets in such a way as to impair acute ACTH stimulation of corticosterone secretion PMID: 12193545
  34. function, structure and genetics of this enzyme in the intestinal mucosa PMID: 12482847
  35. hormone-sensitive lipase translocation requires the phosphorylation of both HSL and perilipin PMID: 12832420
  36. HSL has a role in insulin sensitivity in multiple target tissues of the hormone PMID: 12835327
  37. increased atherosclerosis in hormone-sensitive lipase transgenic mice appears to be due to the coupling of the efficient re-esterification of excess free cholesterol to its limited removal mediated by the cholesterol acceptors in these mice PMID: 14643795
  38. HSL is critically involved in the intracellular processing and availability of cholesterol for adrenal steroidogenesis PMID: 14657254
  39. HSL has roles in adipogenesis as well as in feeding behavior PMID: 14752112
  40. Availability of endogenous free fatty acids through HSL and an additional enzyme(s) is involved in providing lipid moieties for beta-cell signaling for secretion in response to glucose. PMID: 15220197
  41. hormone-sensitive lipase HSLtes has a role in male infertility in mice PMID: 15292223
  42. results suggest that in the absence of HSL, a metabolic switch from reliance on lipid to carbohydrate energy substrates takes place, supporting an important role of HSL in soleus muscle lipid metabolism PMID: 16199803
  43. Perilipin targets a novel pool of lipid droplets for lipolytic attack by hormone-sensitive lipase PMID: 16243839
  44. Beta-cell isoform of HSL is involved in maintaining lipid homeostasis in islets and contributes to the proper control of glucose stimulated insulin secretion. PMID: 16765472
  45. HSL occupies a vital role in adrenal steroidogenesis because of its link to utilization of selectively delivered cholesteryl esters from lipoproteins. PMID: 16985254
  46. These results indicate that the HSL gene is transcriptionally regulated by PPARgamma/RXRalpha heterodimer, and suggest that a cis-acting element regulates the HSL gene expression. PMID: 17134676
  47. Hormone-sensitive lipase-mediated lipolysis and subsequent free fatty acid signaling are components of adipose tissue remodeling following acute and chronic beta3-adrenergic receptor activation. PMID: 17711991
  48. suggests a direct functional role for both HSL and adipose triglyceride lipase in hepatic lipid homeostasis PMID: 18337240
  49. These findings suggest that adipocyte triglyceride lipase and hormone-sensitive lipase differentially modulate biological processes in metabolic tissues. PMID: 18572100
  50. HSL plays a critical role in the hydrolysis of cytosolic cholesteryl esters PMID: 18664600

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Subcellular Location Cell membrane, Membrane, caveola, Cytoplasm, cytosol
Protein Families 'GDXG' lipolytic enzyme family
Database Links

KEGG: mmu:16890

STRING: 10090.ENSMUSP00000003207

UniGene: Mm.158548

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