Code | CSB-E04597m |
Size | 96T,5×96T,10×96T |
Promotion | |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 88 | ||||
Range % | 80-92 | |||||
1:2 | Average % | 92 | ||||
Range % | 88-96 | |||||
1:4 | Average % | 95 | ||||
Range % | 91-105 | |||||
1:8 | Average % | 94 | ||||
Range % | 86-98 |
The recovery of mouse IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-102 | ||||
EDTA plasma (n=4) | 93 | 90-99 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
5000 | 2.597 | 2.654 | 2.626 | 2.478 | |||
2500 | 1.733 | 1.667 | 1.700 | 1.552 | |||
1250 | 1.163 | 1.098 | 1.130 | 0.982 | |||
625 | 0.740 | 0.729 | 0.734 | 0.586 | |||
312 | 0.434 | 0.421 | 0.428 | 0.279 | |||
156 | 0.325 | 0.343 | 0.334 | 0.186 | |||
78 | 0.219 | 0.204 | 0.211 | 0.063 | |||
0 | 0.151 | 0.145 | 0.148 |
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The mouse IL11 Elisa kit is suitable for the quantitative measurement of mouse IL11 in serum, plasma, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of mouse IL11 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, and lot-to-lot consistency.
IL11 binds to its cognate receptor IL11RA, forming the IL11/IL11RA complex that subsequently signals via the gp130 receptor to activate JAK/STAT and ERK pathways to enhance the translation of profibrotic genes. IL11 has been associated with various autoimmune diseases, including rheumatoid arthritis, multiple sclerosis, diabetes, and systemic sclerosis, and has been involved in inflammatory pathologies such as asthma, chronic obstructive pulmonary disease (COPD), inflammatory bowel disease, periodontitis, and psoriasis.
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