Mouse Interleukin 21(IL-21) ELISA Kit

Code CSB-E13162m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9ES17
Lead Time 3-5 working days
Abbreviation IL21
Target Name interleukin 21
Alias IL-21, Za11, interleukin-21 isoform
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 6005
RGD 1307384
MGI 1890474
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %91-101
1:2Average %98
Range %92-103
1:4Average %103
Range %98-108
1:8Average %94
Range %88-98
Recovery
The recovery of mouse IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-97
EDTA plasma (n=4)9692-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.760 2.713 2.737 2.595
5002.431 2.333 2.382 2.240
2501.938 1.912 1.925 1.783
1251.382 1.343 1.363 1.221
62.50.801 0.767 0.784 0.642
31.20.463 0.438 0.451 0.309
15.60.263 0.247 0.255 0.113
00.143 0.141 0.142  
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Function Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
Subcellular Location Secreted
Protein Families IL-15/IL-21 family
Database Links

KEGG: mmu:60505

STRING: 10090.ENSMUSP00000029273

UniGene: Mm.157689

Pathway Jak-STAT signaling pathway
Th17 cell differentiation

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