Mouse Interleukin-36 alpha(IL1F6) ELISA kit

Instructions
Code CSB-EL011617MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 1 family, member 6 (epsilon)
Alternative Names Il36a ELISA Kit; Fil1e ELISA Kit; Il1e ELISA Kit; Il1f6 ELISA Kit; Il1h1Interleukin-36 alpha ELISA Kit; FIL1 epsilon ELISA Kit; Interleukin-1 epsilon ELISA Kit; IL-1 epsilon ELISA Kit; Interleukin-1 family member 6 ELISA Kit; IL-1F6 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit; IL-1H1 ELISA Kit
Abbreviation Il36a
Uniprot No. Q9JLA2
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-90  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 99  
Range % 95-104  
1:8 Average % 93  
Range % 85-99  
Recovery
The recovery of mouse IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-107  
EDTA plasma (n=4) 88 80-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.591 2.587 2.589 2.486  
500 2.017 2.046 2.032 1.929  
250 1.365 1.389 1.377 1.274  
125 0.799 0.751 0.775 0.672  
62.5 0.455 0.483 0.469 0.366  
31.2 0.261 0.243 0.252 0.149  
15.6 0.169 0.186 0.178 0.075  
0 0.102 0.104 0.103    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. Induces the production of proinflammatory cytokines, including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23 in bone marrow-derived dendritic cells (BMDCs). Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes. May play a role in proinflammatory effects in the lung
Gene References into Functions
  1. The data identify a novel role for IL-36 signaling in colonic inflammation and indicate that the IL-36R pathway may represent a novel target for therapeutic intervention. PMID: 26813344
  2. IL-1 and IL-36 have roles as inflammatory mediators in pustular psoriasis in a mouse model PMID: 28057979
  3. IL-1alpha and IL-36alpha form a self-amplifying inflammatory loop in vivo that in patients with insufficient counter regulatory mechanisms may become hyper-engaged and/or chronic PMID: 26203636
  4. An essential role of C/EBPbeta in the regulation of the Il36A gene via the proximal half-CRE*C/EBP element in response to inflammatory stimuli. PMID: 26066982
  5. IL-36 promotes myeloid cell infiltration, activation, and inflammatory activity in skin PMID: 24829417
  6. IL-36alpha acts as a pro-inflammatory cytokine in the lungs independent of both IL-1alpha and IL-1beta. PMID: 23029241
  7. Interleukin-36 (IL-36) ligands require processing for full agonist (IL-36alpha, IL-36beta, and IL-36gamma) or antagonist (IL-36Ra) activity PMID: 21965679
  8. A critical role of IL-36R ligands in the interface between innate and adaptive immunity, leading to the stimulation of T helper responses. PMID: 21860022
  9. Transcripts for IL-1F5, -1F6, -1F8, and -1F9 are all significantly increased in the involved skin of bitransgenic mice compared with their monotransgenic controls. PMID: 21242515
  10. local overexpression related to the development of tubulointerstitial lesions PMID: 20101239
  11. IL-1F6 can be externalized via a stimulus-coupled mechanism comparable to that used by IL-1beta PMID: 19717513

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Subcellular Location Secreted
Protein Families IL-1 family
Tissue Specificity Highly expressed in embryonic tissue and in tissues containing epithelial cells. Elevated expression levels are detected in chronic kidney disease; expressed inepithelia from the distal convoluted tubules (DCTs) to the cortical collecting ducts (CCDs) in
Database Links

KEGG: mmu:54448

STRING: 10090.ENSMUSP00000028361

UniGene: Mm.133095

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