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Recombinant Mouse Interleukin-36 alpha protein (Il36a) (Active)

Recombinant Mouse Interleukin-36 alpha protein (Il36a) (Active)

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Code	CSB-AP003421MO
Abbreviation	Recombinant Mouse Il36a protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Product Details
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Target Background

Product Details

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rMuIL-36α at 1 ug/mL can bind recombinant murine IL-1 Rrp2 with a range of 0.15-5 ug/mL.

Target Names

Il36a

Uniprot No.

Q9JLA2

Research Area

Immunology

Alternative Names

Il36a; Fil1e; Il1e; Il1f6; Il1h1Interleukin-36 alpha; FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6; Interleukin-1 homolog 1; IL-1H1

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-160aa

Complete Sequence

MNKEKELRAA SPSLRHVQDL SSRVWILQNN ILTAVPRKEQ TVPVTITLLP CQYLDTLETN RGDPTYMGVQ RPMSCLFCTK DGEQPVLQLG EGNIMEMYNK KEPVKASLFY HKKSGTTSTF ESAAFPGWFI AVCSKGSCPL ILTQELGEIF ITDFEMIVVH

Mol. Weight

18.0 kDa

Protein Length

Full Length

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 5 % trehalose

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

IL-36α (IL-1F6) signals through the IL-1Rrp2/IL-1RAcP receptor complex to drive NF-κB and MAPK activation, making it a key mediator in epithelial and immune cell inflammatory responses. This tag-free recombinant mouse IL-36α encompasses the full-length sequence (1–160 aa) and demonstrates confirmed binding to recombinant murine IL-1Rrp2 across a 0.15–5 µg/mL range when immobilized at 1 µg/mL in functional ELISA, supporting its use as a coating antigen for antibody validation, a reference standard in cytokine detection assays, and a reliable ligand in receptor-binding studies. Endotoxin levels below 1.0 EU/µg minimize LPS-driven artifacts in sensitive cell-based assays such as NF-κB reporter activation, keratinocyte or immune cell stimulation, and in vivo murine inflammation models. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin control, provides a suitable basis for downstream signaling pathway dissection and antibody development workflows.

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Target Background

Function

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. Induces the production of proinflammatory cytokines, including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23 in bone marrow-derived dendritic cells (BMDCs). Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes. May play a role in proinflammatory effects in the lung: induces the expression of CXCL1 and CXCL2 in the lung, and the expression of TNF-alpha, IL-36c, IL-1A, IL-1B, CXCL1 and CXCL2 in isolated splenic CD11c(+) alveolar macrophages. May be involved in T-cell maturation by stimulating the surface expression of CD40 and modestly CD80 and CD86 in splenic CD11c(+) cells. May be involved in CD4(+) T-cell proliferation. Induces NF-kappa B activation in macrophages.

Gene References into Functions

The data identify a novel role for IL-36 signaling in colonic inflammation and indicate that the IL-36R pathway may represent a novel target for therapeutic intervention. PMID: 26813344
IL-1 and IL-36 have roles as inflammatory mediators in pustular psoriasis in a mouse model PMID: 28057979
IL-1alpha and IL-36alpha form a self-amplifying inflammatory loop in vivo that in patients with insufficient counter regulatory mechanisms may become hyper-engaged and/or chronic PMID: 26203636
An essential role of C/EBPbeta in the regulation of the Il36A gene via the proximal half-CRE*C/EBP element in response to inflammatory stimuli. PMID: 26066982
IL-36 promotes myeloid cell infiltration, activation, and inflammatory activity in skin PMID: 24829417
IL-36alpha acts as a pro-inflammatory cytokine in the lungs independent of both IL-1alpha and IL-1beta. PMID: 23029241
Interleukin-36 (IL-36) ligands require processing for full agonist (IL-36alpha, IL-36beta, and IL-36gamma) or antagonist (IL-36Ra) activity PMID: 21965679
A critical role of IL-36R ligands in the interface between innate and adaptive immunity, leading to the stimulation of T helper responses. PMID: 21860022
Transcripts for IL-1F5, -1F6, -1F8, and -1F9 are all significantly increased in the involved skin of bitransgenic mice compared with their monotransgenic controls. PMID: 21242515
local overexpression related to the development of tubulointerstitial lesions PMID: 20101239
IL-1F6 can be externalized via a stimulus-coupled mechanism comparable to that used by IL-1beta PMID: 19717513

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Subcellular Location

Cytoplasm. Secreted.

Protein Families

IL-1 family

Tissue Specificity

Highly expressed in embryonic tissue and in tissues containing epithelial cells. Elevated expression levels are detected in chronic kidney disease; expressed inepithelia from the distal convoluted tubules (DCTs) to the cortical collecting ducts (CCDs) in

Database Links

KEGG: mmu:54448

STRING: 10090.ENSMUSP00000028361

UniGene: Mm.133095

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