Mouse Interleukin 3,IL-3 ELISA KIT

Code CSB-E04632m
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 3 (colony-stimulating factor, multiple)
Alternative Names
Il3 ELISA Kit; Csfmu ELISA Kit; Il-3 ELISA Kit; Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
Abbreviation
IL3
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 83-92
1:2 Average % 95
Range % 91-100
1:4 Average % 90
Range % 86-99
1:8 Average % 95
Range % 86-98
Recovery
The recovery of mouse IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-98
EDTA plasma (n=4) 97 86-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.695 2.680 2.688 2.511
250 1.885 1.867 1.876 1.699
125 1.302 1.295 1.299 1.122
62.5 0.758 0.744 0.751 0.574
31.2 0.499 0.492 0.496 0.319
15.6 0.356 0.359 0.358 0.181
7.8 0.281 0.287 0.284 0.107
0 0.175 0.179 0.177  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse IL3 ELISA Kit was designed for the quantitative measurement of Mouse IL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

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Target Background

Function
(From Uniprot)
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Gene References into Functions
  1. Jak1-deficient hematopoietic stem cells exhibit increased quiescence, an inability to enter the cell cycle in response to hematopoietic stress, and a marked reduction in cytokine sensing, including in response to type I interferons and IL-3. PMID: 28965767
  2. findings indicate that STAT5 contributes to the remarkable IL-3-mediated inhibition of RANKL-induced osteoclastogenesis by activating Id genes and their associated pathways. PMID: 27485735
  3. c-Kit(+) Adipose tissue-derived mesenchymal stem cells (ASCs)may promote breast cancer growth and angiogenesis by a synergistic effect of c-Kit and IL-3. Our findings suggest that c-Kit(+) subpopulations of ASCs should be eliminated in fat grafts for breast reconstruction of cancer patients following mastectomy. PMID: 28573141
  4. These impaired macrophage functions in leukemic mice were significantly corrected by IL-3 and GM-CSF treatment indicating the therapeutic benefit of these two cytokines in leukemia. PMID: 28039843
  5. IL-3 signaling does not contribute to Jak2 V617F myeloproliferative neoplasm pathogenesis. PMID: 26589916
  6. Thus, IL-3 plays an important role in the pathogenesis of SLE and the progression of lupus nephritis. PMID: 26131743
  7. Cytoplasmic granule containing HERMES, NonO, PSF, and G3BP1 is a neuronal RNA-protein granule that is transported in neurites during retinal differentiation. PMID: 25651939
  8. Stem cell factor (SCF), but not interleukin-3 (IL-3), is a major effector of HSC maturation during embryonic day E9-E10. PMID: 25241746
  9. study reports IL3 potentiates inflammation in sepsis; in model of abdominal sepsis, findings show innate response activator B cells produce IL3, which induces myelopoiesis of monocytes and neutrophils and fuels cytokine storm; IL3 deficiency protects against sepsis PMID: 25766237
  10. Altered expression of CD30L and IL-3 may be potential biomarkers for hepatotoxicity induced by D. bulbifera. PMID: 24647110
  11. This study is the first to link beta-catenin activation to IL-3 and suggests that targeting IL-3 signaling may be an effective approach for the inhibition of beta-catenin activity in some patients with AML. PMID: 24598054
  12. IL-3 plays a critical role in suppressing protective immunity to P. berghei NK65 infection. PMID: 24379292
  13. IL-3 promotes Stat5 activation in osteoclasts. PMID: 24367002
  14. these findings not only provide a better understanding of the role of IL-3 in osteoclastogenesis but may also facilitate future studies to delineate the role of IL-3 in the pathogenesis of bone diseases. PMID: 24103757
  15. At day 10, CIP treatment not only significantly reduced pro-inflammatory cytokine and chemokine concentrations, including interleukin-6 (IL-6) and KC, but it also enhanced IL-3 production compared to vehicle-treated controls. PMID: 23520506
  16. IL-3 upregulates Trib3 mRNA expression in bone-marrow-derived mast cells. During prolonged IL-3 starvation, cell death is accelerated in Trib3-null cultures. PMID: 23261831
  17. confirm for the first time that IL-3 and IL-4 are critical for IL-33 intracrine in murine cells of various types, indicating that IL-3 and IL-4 may play an important role in the constitutive expression of IL-33 in vivo PMID: 22370606
  18. p53(-/-) cells have a deregulated intracellular signaling environment and display a more rapid and sustained response to IL-3. This was accompanied by an increase in active ERK1/2 and a dependence on an intact MAP kinase signaling pathway PMID: 22348085
  19. Mast cells cultured from IL-3-treated mice show impaired responses to bacterial antigen stimulation PMID: 22068549
  20. these findings suggest CNSa is a distal enhancer of the IL-3/GM-CSF gene cluster that binds BRG1 and NF-kappaB PMID: 21831442
  21. IL-3 markedly amplifies primitive erythroid and macrophage precursors in E7.5 embryos and has a regulatory role with regard to both number and capacity of the dual-potential hemangioblast. PMID: 20007140
  22. Inhibition of IL-3 signaling and knockdown of Xbp1-induced apoptosis in hematopoietic cells. PMID: 21368889
  23. Data indicate a role for PLCgamma2 and Ca(2+) signaling through the modulation of MEK/ERK in IL3/GM-csf stimulated mouse hematopoietic stem/progenitor cells. PMID: 21506110
  24. structural studies of soluble, recombinant IL3 fragment (33-156): four-helical bundle fold; conformation typical of short-chain cytokines; core of highly conserved hydrophobic residues; pronounced conformational heterogeneity PMID: 21329364
  25. Complex interactions in EML cell stimulation by stem cell factor and IL-3. PMID: 21383156
  26. Recombinant murine IL-3 plays an important role in modulating regulatory T (Treg) cell development in both in vitro and in vivo conditions and significantly reduces the severity of collagen-induced arthritis. PMID: 21242512
  27. Studies indicate that BMP and IL-3 signaling pathways are critical for the growth and potential of embryonic HSCs. PMID: 20711995
  28. SHP-1 positively regulates IL-3-dependent mast cell proliferation and apoptosis by inhibiting ERK activity through its phosphatase activity. PMID: 21044800
  29. IL-3 irreversibly inhibits RANK expression that results in inhibition of important signaling molecules induced by RANKL during osteoclastogenesis. PMID: 20691668
  30. the domain 1 D-E loop disulfide of hbetac and beta(IL-3) in maintaining the precise positions of ligand-binding residues necessary for normal high affinity binding and signaling PMID: 20516062
  31. Two different modes of beta c binding are utilized in the presence of the hIL-3R alpha isoforms. PMID: 20472554
  32. The IL-3/IL-3 receptor system is absolutely required to recruit circulating basophils into the draining lymph nodes following helminth infection. PMID: 20038645
  33. IL-3 and oncogenic Abl regulate the myeloblast transcriptome by altering mRNA stability PMID: 19829692
  34. p53 protein is activated after IL-3 deprivation by loss of MDM2. Activated p53 transcriptionally up-regulates Puma, which initiates apoptosis. PMID: 19965665
  35. Role of pRB-family/E2F complex in the inhibition of IL-3-dependent lymphoid cell proliferation. PMID: 11886176
  36. relevance to peripheral myeloid recruitment PMID: 12115609
  37. IL-3-driven survival and proliferation is negatively regulated, potentially via tyrosine phosphorylation of Aic2A and STAT5 PMID: 12220225
  38. IL3 is required for mitochondrial respiratory control PMID: 12228733
  39. evidence that IL-3 production is a rapid, sustained, and biologically relevant consequence of BCR-ABL expression in primitive hematopoietic cells with multilineage leukemogenic activity PMID: 12393460
  40. IL-3 induces activation of the PI-3 kinase, MAP kinase, & Jak/Stat pathways. Jak2 activation is the critical "proximal" mediator of the IL-3-induced enhancement of RAR activity. PMID: 12393611
  41. IL-3 inhibits osteoclastogenesis in whole bone marrow cells by directly acting on osteoclast precursors, irreversibly blocking receptor activator of NF-kappaB ligand (RANKL)-induced osteoclast differentiation by diverting the cells to macrophage lineage. PMID: 12816992
  42. The time courses for activation of phosphatidylinositol 3-kinase and its downstream target, protein kinase B, by IL-3 were consistent with a role in IL-3-induced transporter translocation and enhanced glucose uptake. PMID: 12869574
  43. Interleukin-3 and flt3 ligand induce expression of antiapoptotic Bcl-2 family genes. PMID: 12960281
  44. Interleukin-3 binding to the murine betaIL-3 receptor involves functional epitopes formed by domains 1 and 4 of different protein chains PMID: 15060062
  45. IL-3 plays a crucial role for IgE(-Ag)-induced mast cell survival, functioning in an autocrine manner by inducing the Bcl-xL/Bcl-2 via signal transducer and activator of transduction 5 PMID: 15542585
  46. IL-3 induces inhibitor of DNA-binding protein 1 (Id1) expression in multipotential erythroid-myeloid-lymphoid cells during myeloid, but not B cell or erythroid cell differentiation. PMID: 15905544
  47. Results describe the effects of a leukemia-associated gain-of-function mutation of SHP-2 phosphatase on interleukin-3 signaling. PMID: 16371368
  48. The importance of IL-3-induced TNF secretion was demonstrated by the failure of TNF-deficient bone marrow cells to survive for >3 wk when cultured in IL-3 and SCF, a defect that was reversed by the addition of soluble TNF PMID: 16455967
  49. Inhibition of the PI3 kinase pathway promoted apoptosis in the presence or absence of IL-3. PMID: 16705087
  50. in mice with markedly impaired SCF/c-Kit signaling, IL-3 contributed significantly to the increased numbers of eosinophils that were observed during Strongyloides venezuelensis infection, but not during infection with Nippostrongylus brasiliensis PMID: 16894356

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Subcellular Location
Secreted.
Protein Families
IL-3 family
Tissue Specificity
Activated T-cells, mast cells, natural killer cells.
Database Links

KEGG: mmu:16187

STRING: 10090.ENSMUSP00000019058

UniGene: Mm.983

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