Interleukin-3 is a protein in humans that is encoded by IL3 gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; FUNCTION
The following IL3 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
IL3 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA011652GA01HU | IL3 Antibody |
Human | ELISA,WB |
CSB-PA06725B0Rb | IL3 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA06725A0Rb | IL3 Antibody |
Human | ELISA, IHC |
CSB-PA011652ESR1HU | IL3 Antibody |
Human | ELISA, IHC |
CSB-PA196621 | IL3 Antibody |
Human,Mouse | ELISA,WB |
IL3 Proteins for Macaca mulatta (Rhesus macaque)
Code | Product Name | Source |
---|---|---|
CSB-YP011652MOW CSB-EP011652MOW CSB-BP011652MOW CSB-MP011652MOW CSB-EP011652MOW-B |
Recombinant Macaca mulatta (Rhesus macaque) Interleukin-3(IL3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-AP001981MOW | Recombinant Rhesus Macaque Interleukin-3 protein(IL3) (Active) |
E.coli |
IL3 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP011652BO CSB-EP011652BO CSB-BP011652BO CSB-MP011652BO CSB-EP011652BO-B |
Recombinant Bovine Interleukin-3(IL3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IL3 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP011652MO CSB-EP011652MO CSB-BP011652MO CSB-MP011652MO CSB-EP011652MO-B |
Recombinant Mouse Interleukin-3(Il3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-AP003291MO | Recombinant Mouse Interleukin-3 protein(Il3),partial (Active) |
E.Coli |
IL3 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP011652RA CSB-EP011652RA CSB-BP011652RA CSB-MP011652RA CSB-EP011652RA-B |
Recombinant Rat Interleukin-3(Il3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IL3 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP011652HU CSB-BP011652HU CSB-MP011652HU CSB-EP011652HU-B |
Recombinant Human Interleukin-3(IL3) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP011652HU | Recombinant Human Interleukin-3(IL3) |
E.coli |
CSB-AP001701HU | Recombinant Human Interleukin-3 protein(IL3) (Active) |
E.coli |
CSB-EP011652HUc7 | Recombinant Human Interleukin-3(IL3) |
E.coli |
CSB-AP004521HU | Recombinant Human Interleukin-3(IL3) (Active) |
Mammalian cell |
CSB-AP004531HU | Recombinant Human Interleukin-3(IL3) (Active) |
E.coli |
CSB-AP005821HU | Recombinant Human Interleukin-3(IL3),partial (Active) (GMP) |
E.Coli |
IL3 Proteins for Saguinus oedipus (Cotton-top tamarin)
Code | Product Name | Source |
---|---|---|
CSB-YP011652SWB CSB-EP011652SWB CSB-BP011652SWB CSB-MP011652SWB CSB-EP011652SWB-B |
Recombinant Saguinus oedipus Interleukin-3(IL3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IL3 Proteins for Hylobates lar (Common gibbon) (White-handed gibbon)
Code | Product Name | Source |
---|---|---|
CSB-YP011652HXW CSB-EP011652HXW CSB-BP011652HXW CSB-MP011652HXW CSB-EP011652HXW-B |
Recombinant Hylobates lar Interleukin-3(IL3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IL3 Proteins for Callithrix jacchus (White-tufted-ear marmoset)
Code | Product Name | Source |
---|---|---|
CSB-YP632965CYL CSB-EP632965CYL CSB-BP632965CYL CSB-MP632965CYL CSB-EP632965CYL-B |
Recombinant Callithrix jacchus Interleukin-3(IL3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IL3 Proteins for Pan troglodytes (Chimpanzee)
Code | Product Name | Source |
---|---|---|
CSB-YP637919EQV CSB-EP637919EQV CSB-BP637919EQV CSB-MP637919EQV CSB-EP637919EQV-B |
Recombinant Pan troglodytes Interleukin-3(IL3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IL3 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Source |
---|---|---|
CSB-YP860200DO CSB-EP860200DO CSB-BP860200DO CSB-MP860200DO CSB-EP860200DO-B |
Recombinant Dog Interleukin-3(IL3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IL3 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04631h | Human Interleukin 3,IL-3 ELISA KIT |
serum, plasma, tissue homogenates. | 15.6 pg/ml. |
IL3 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04632m | Mouse Interleukin 3,IL-3 ELISA KIT |
serum, plasma, tissue homogenates | 1.95 pg/mL |
IL3 ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E07436r | Rat Interleukin 3,IL-3 ELISA KIT |
serum, plasma, tissue homogenates | 0.78 pg/mL |
Interleukin-3 (IL3) is a multipotent cytokine produced by activated T cells, monocytes/macrophages, and stroma cells that links immunity to the hemopoietic system and plays a remarkable role in leukemia as well as several immunopathologies [1]. Through actions on several cell types, IL3 contributes to allergic inflammation, autoimmune diseases, and oncogenesis [2][3]. The downstream effects of IL3 are mediated by binding to its receptor, a complex that contains an alpha subunit (IL3R or CD123) and a beta subunit (IL3Rβ or CSF2RB) [4]. Upon IL3 stimulation, a cascade of signaling mechanisms is elicited, including activation of the JAK2/STAT5 pathway and the PI3 kinase pathway [5]. Further, activation of the NF-kappa B pathway through TRAF6 recruitment to the beta subunit has been described [6]. These pathways not only leads to the cell-cycle progression and DNA synthesis but also suppression of apoptosis, thus facilitating cellular survival against apoptosis. IL3 is also involved in bone marrow hemopoiesis and dendritic cell maturation in anti-viral or antitumor reactivity. Since IL-3 is constitutively present in the myelomonocytic leukemia cell line WEHI-3B, it is thought that this genetic change is the key to the development of this leukemia type [7]. It has been shown that the combination of IL-3, GM-CSF, and stem cell factor enhances peripheral blood stem cells during high-dose chemotherapy [8][9]. Post-chemotherapy IL-3 application reduces chemotherapy delays and promotes regeneration of granulocytes and platelets. However, only IL-3 treatment in bone marrow failure disorders such as myelodysplastic syndrome (MDS) and aplastic anemia (AA) was disappointing [10].
[1] Hercus T.R., Dhagat U., et al. Signalling by the bc family of cytokines [J]. Cytokine Growth Factor Rev. 2013, 24, 189-201.
[2] Korpelainen E.I., Gamble J.R., et al. The receptor for interleukin 3 is selectively induced in human endothelial cells by tumor necrosis factor alpha and potentiates interleukin 8 secretion and neutrophiltransmigration [J]. Proc. Natl. Acad. Sci. USA 1993, 90, 11137-11141.
[3] Broughton S.E., Dhagat U., et al. The GM-CSF/IL-3/IL-5 cytokine receptor family: from ligand recognition to initiation of signaling [J]. Immunol. Rev. 2012, 250, 277-302.
[4] Takai S, Yamada K, et al. Mapping of the human gene encoding the mutual signal-transducing subunit (beta-chain) of granulocyte-macrophage colonystimulating factor (GM-CSF), interleukin-3 (IL-3), and interleukin-5 (IL-5) receptor complexes to chromosome 22q13.1 [J]. Hum Genet 1994;93(2):198-200.
[5] Hercus TR et al. Role of the β common (βc) family of cytokines in health and disease [J]. Cold Spring Harb Perspect Biol 2018;10(6).
[6] Meads MB, Li Z-W, et al. A novel TNF receptor-associated factor 6 binding domain mediates NF-kappa B signaling by the common cytokine receptor beta subunit [J]. J Immunol 2010;185(3):1606-1615.
[7] Aiguo, Wu, Guangren, Duan. PMID Observer Design of Descriptor Linear Systems [J]. 2007 Chinese Control Conference. 2016, IEEE: 161-165.
[8] Serrano F, Varas F, et al. Accelerated and longterm hematopoietic engraftment in mice transplanted with ex-vivo expanded bone marrow [J]. Bone Marrow Transplant. 1996, 14 (6): 855-62.
[9] Peters SO, Kittler EL, et al. Ex-vivo expansion of murine marrow cells with IL-3, Il-6, Il-11 and SCF leads to impaired engraftment in irradiated host [J]. Blood. 1996, 87 (1): 30-7.
[10] Manzoor H Mangi, Adrian C Newland. Interleukin-3 in hematology and onkology: Current state of knowledge and future directions [J]. Cytokines, Cellular and Molecular Therapy. 5: 87-95.