Mouse Peroxiredoxin-1(PRDX1) ELISA kit

Code CSB-EL018653MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P35700
Lead Time 7-14 working days
Abbreviation PRDX1
Target Name peroxiredoxin 1
Alias MSP23, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2, natural killer-enhancing factor A|proliferation-associated gene A|thioredoxin-dependent peroxide reductase 2
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRDX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-94  
1:2 Average % 98  
Range % 92-104  
1:4 Average % 102  
Range % 98-106  
1:8 Average % 94  
Range % 90-98  
Recovery
The recovery of mouse PRDX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 93-101  
EDTA plasma (n=4) 94 91-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.788 2.652 2.720 2.539  
10 1.764 1.693 1.729 1.548  
5 1.110 1.063 1.087 0.906  
2.5 0.721 0.689 0.705 0.524  
1.25 0.366 0.387 0.377 0.196  
0.625 0.293 0.276 0.285 0.104  
0.312 0.229 0.235 0.232 0.051  
0 0.179 0.182 0.181    
References
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Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation
Subcellular Location Cytoplasm
Protein Families Peroxiredoxin family, AhpC/Prx1 subfamily
Tissue Specificity Found in various tissues; high concentration in liver.
Database Links

KEGG: mmu:18477

STRING: 10090.ENSMUSP00000102078

UniGene: Mm.30929

Pathway Cell ageing and metabolism

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