Mouse Plasminogen(PLG) ELISA kit

Instructions
Code CSB-EL018188MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name plasminogen
Alternative Names Plg ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
Abbreviation PLG
Uniprot No. P20918
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates_x000D_
Detection Range 62.5 ng/mL-4000 ng/mL
Sensitivity 15.63 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 98    
Range % 92-103    
1:200 Average % 97    
Range % 93-102    
1:400 Average % 104    
Range % 98-108    
1:800 Average % 86    
Range % 81-92    
Recovery
The recovery of mouse PLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 87 83-93    
EDTA plasma (n=4) 94 88-98    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
4000 0.098 0.099 0.099      
2000 0.125 0.132 0.129      
1000 0.179 0.184 0.182      
500 0.290 0.308 0.299      
250 0.462 0.475 0.469      
125 0.758 0.788 0.773      
62.5 1.015 1.103 1.059      
0 2.002 2.110 2.056      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity).
Gene References into Functions
  1. These studies suggest a crucial role for peripheral PL in mediating neuroimmune cell activation and Alzheimer's disease (AD) progression and could provide a link to systemic inflammatory risk factors that are known to be associated with AD development. PMID: 30254165
  2. Variation in blood plasminogen levels, within the range seen in normal individuals, had marked effects on experimental ischemic brain injury PMID: 27319402
  3. plasmin protease activity prevents dystrophic calcification within injured skeletal muscle independent of its canonical fibrinolytic function. PMID: 27530373
  4. Plasminogen-deficient mice have extensive fibrin and neutrophil depositions in the wounded area long after re-epithelialisation, indicating inefficient debridement and chronic inflammation. Delayed formation of granulation tissue suggests that fibroblast function is impaired in the absence of plasminogen. PMID: 26791370
  5. findings identify plasmin as a decisive checkpoint in the inflammatory response during Macrophage activation syndrome (MAS) and a potential novel therapeutic target for MAS. PMID: 28325863
  6. Deficiency of plasminogen delays onset of autoimmune neuroinflammatory disease and protects from the development of paralysis. PMID: 28275164
  7. Plasminogen may regulate DPP-4 activity and glucose metabolism. PMID: 27592166
  8. Plasminogen-deficient mice frequently develop colon lesions and rectal prolapses. PMID: 26473342
  9. manganese transport protein C (MntC) is an extracellular matrix- and plasminogen-binding protein PMID: 25409527
  10. Plg from mouse plasma contains oxPtdPC adducts that are not affected by the action of Lp-PLA(2), suggesting that linkage to Plg protects oxPtdPCs from metabolism during their transport in the plasma. PMID: 21614211
  11. plasminogen-dependent proteolysis has a beneficial effect during neurological recovery after stroke. PMID: 24732409
  12. Pla-induced mononuclear cell recruitment in vivo was dependent on protease-activated receptor-1 activation of the MEK/ERK/NF-kappaB pathway, which led to the release of CCL2 and activation of CCR2. PMID: 25165151
  13. Plg may play an important role in innate immunity by changing expression of genes that contribute to phagocytosis. PMID: 24876560
  14. Plasminogen is a key molecular determinant of inflammatory joint disease capable of simultaneously driving or ameliorating arthritis pathogenesis in distinct anatomic locations in the same subject. PMID: 24574269
  15. Plasmin suppresses brain metastasis in two ways: by converting membrane-bound astrocytic FasL into a paracrine death signal for cancer cells, and by inactivating the axon pathfinding molecule L1CAM, which metastatic cells express for spreading along brain capillaries and for metastatic outgrowth. PMID: 24581498
  16. Plasminogen stimulates autocrine cytokine production in human airway smooth muscle cells in a manner mediated by plasmin and annexin A2. PMID: 23721211
  17. plasminogen contributes to angiogenesis related to macrophage accumulation, TGF-beta, and VEGF, thereby leading to the enhancement of bone repair. PMID: 23456978
  18. These findings highlight a critical role for plasmin in the catabolism of aggregated LDL by macrophages and provide a new context for considering the atherogenic role of plasmin. PMID: 23702659
  19. Gender affects skin wound healing in plasminogen deficient mice. PMID: 23527289
  20. The increased plasmin generation during the early stages of sepsis could cleave/inactivate TFPI and thus lead to thrombotic complications. PMID: 23106863
  21. Macrophage gene expression and foam cell formation are regulated by plasminogen. PMID: 23401155
  22. These results demonstrate the novel requirement of a-enolase for restoring homeostasis of injured muscle tissue, by controlling the pericellular localization of plasmin activity. PMID: 23239981
  23. In summary, these data indicate that plasmin-driven fibrinolysis facilitates tumor growth by maintaining patency of the tumor vasculature. PMID: 22815383
  24. Plasmin is essential in preventing periodontitis in mice PMID: 21704601
  25. found that male Plg knockout mice, but not their female counterparts, exhibited normochromic normocytic anemia PMID: 22056679
  26. Dengue virus (DENV)-immunized mouse sera demonstrates antibody binding to both DENV and Plg when compared with nonimmune control mouse serum. PMID: 22079981
  27. Plasmin plays an essential role in amplification of psoriasiform skin inflammation. PMID: 21311769
  28. in endothelial cells, plasmin specifically induces activation of conventional PKC, which phosphorylates serine 11 and serine 25 of A2, triggering dissociation of the (A2.p11)(2) tetramer. PMID: 21115493
  29. Plasminogen is highly expressed in specific populations of hippocampal, cortical, and hypothalamic neurons, and plasminogen-containing vesicles are mainly observed at neuronal somata. PMID: 21190118
  30. Plasminogen/plasmin modulates bone metabolism by regulating the osteoblast and osteoclast function. PMID: 21239499
  31. analysis of the generation and characterization of mice expressing a plasmin-inactivating active site mutation PMID: 20653841
  32. Data show that upon binding of plasminogen to neurons, plasmin formed by constitutively expressed tissue plasminogen activator degrades extracellular matrix proteins, leading to retraction of the neuron monolayer that detaches from the matrix. PMID: 19683575
  33. The role of Plg and alpha2-AP in vascular thrombolysis was studied in mice lacking Plg or a2-AP. Lack of Plg reduces the antithrombotic activities in vivo, but alpha2-AP plays a more important role in the formation/removal of venous thrombi. PMID: 11848464
  34. RT-PCR and In Situ Hybridization study of tissue distribution of Plg mRNA in adrenal, kidney, brain, testis, heart, lung, uterus, spleen, thymus and gut PMID: 11916082
  35. Plasmin activity is required for myogenesis in vitro and skeletal muscle regeneration in vivo. PMID: 11929773
  36. important role in adipose tissue accumulation associated with growth and differentiation of stromal cells PMID: 12083480
  37. These results indicate the presence of regulatory elements in the 5'-flanking region of the murine plasminogen promoter that may regulate murine plasminogen gene expression and, hence, plasmin activity. PMID: 12149246
  38. Plg mRNA was undetectable in the normal brain, but after kainate injection it was induced in neuronal cells in multiple, but specific areas. This distribution pattern coincided with that of the plasminogen activator inhibitor-2 mRNA. PMID: 12225871
  39. role of pCPB as a modulator of the pivotal functions of plasminogen in fibrinolysis and cell migration in vivo. PMID: 12417566
  40. Significantly greater retention of PMNs within the thrombi of PG(-/-) mice provided potential for more PMN derived fibrinolytic enzymes and a relatively greater role for PMNs-initiated fibrinolysis and differently cleaved fibrinogen degradation products. PMID: 12428098
  41. plasminogen directs the hepatocyte injury induced by transgene-derived urokinase-type plasminogen activator and mediates the reparative properties of urokinase-type plasminogen activator in the liver PMID: 12431907
  42. plasminogen mRNA from hepatocyte is dependent on the cell density and the stimulation by cell-cell contact may be an important factor for the constitutive expression of plasminogen gene PMID: 12559391
  43. Results demonstrate that the combination of matrix metalloproteinases and plasminogen is essential for the proper development of the placenta. PMID: 12900459
  44. plasmin has a role as an intracellular protease PMID: 12928439
  45. When uPA and uPAR transgenic mice were backcrossed into a plasminogen-deficient (Plg-/-) background, the dental and skin phenotypes appeared completely normal. PMID: 15161662
  46. Corneal angiogenesis is dependent on Plg. PMID: 15166500
  47. Plasminogen is bound to the cell surface by histidine-rich glycoprotein PMID: 15220341
  48. Plasminogen exerts a marked influence on both neutrophil and monocyte/macrophage recruitment to implanted biomaterials. PMID: 15456492
  49. Plg/Pla stimulates c-fos and egr-1 expression via activation of the MEK/ERK pathway PMID: 15721299
  50. Our results suggest that plasmin-mediated cell detachment could contribute to the pathological effects of Abeta in diseased brain. PMID: 15737740

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Subcellular Location Secreted
Protein Families Peptidase S1 family, Plasminogen subfamily
Database Links

KEGG: mmu:18815

STRING: 10090.ENSMUSP00000014578

UniGene: Mm.971

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