Mouse Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Code CSB-E06882m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

The mouse prolactin (PRL) ELISA Kit is used to quantitatively measure PRL concentrations in mouse serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the competitive ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops inversely to the amount of PRL in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

PRL is a sex hormone that is mainly synthesized and secreted by the lactotrophs of the pituitary and is responsible for the induction of lactation, the development of mammary alveoli, and milk production. It also exerts immunoregulatory properties. PRL signals by binding to PRL and activates a signaling cascade, including Jak/Stat, MAPK, Src, and PI3K/Akt pathways. PRL levels normally increase during pregnancy and breastfeeding and may rise due to physical stress, exercise, and certain medications and diseases. Higher-than-normal levels of PRL can cause certain symptoms, such as irregular periods, infertility, and erectile dysfunction.

Target Name prolactin
Alternative Names Prl ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
Abbreviation PRL
Uniprot No. P06879
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.8 ng/mL-28 ng/mL
Sensitivity 0.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-101  
1:2 Average % 93  
Range % 90-96  
1:4 Average % 95  
Range % 89-102  
1:8 Average % 96  
Range % 90-102  
Recovery
The recovery of mouse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-100  
EDTA plasma (n=4) 96 89-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
25 0.165 0.174 0.170    
4.69 0.352 0.374 0.363    
0.78 0.573 0.589 0.581    
0.22 1.026 1.048 1.037    
0.06 1.562 1.574 1.568    
0 2.205 2.233 2.219    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Prolactin acts primarily on the mammary gland by promoting lactation.
Subcellular Location Secreted.
Protein Families Somatotropin/prolactin family
Database Links

STRING: 10090.ENSMUSP00000105998

UniGene: Mm.1270

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