Mouse Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Code CSB-E06882m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P06879
Lead Time 3-5 working days
Abbreviation PRL
Protein Biological Process 1 fertilization
Target Name prolactin
Alias N/A
Species Mus musculus (Mouse)
Protein Biological Process 3 Lactation
Sample Types serum, plasma, tissue homogenates
Detection Range 0.8 ng/mL-28 ng/mL
Sensitivity 0.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
HGNC 9089
RGD 621047
MGI 1858197
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-101  
1:2 Average % 93  
Range % 90-96  
1:4 Average % 95  
Range % 89-102  
1:8 Average % 96  
Range % 90-102  
Recovery
The recovery of mouse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-100  
EDTA plasma (n=4) 96 89-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
25 0.165 0.174 0.170    
4.69 0.352 0.374 0.363    
0.78 0.573 0.589 0.581    
0.22 1.026 1.048 1.037    
0.06 1.562 1.574 1.568    
0 2.205 2.233 2.219    
References
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Function Prolactin acts primarily on the mammary gland by promoting lactation.
Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

STRING: 10090.ENSMUSP00000105998

UniGene: Mm.1270

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway

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