Recombinant Mouse Prolactin (Prl) (Active)

Prolactin drives JAK2/STAT5 signaling through the prolactin receptor, making validated bioactivity essential for meaningful downstream results. This tag-free recombinant mouse Prl, covering the full mature sequence (residues 30–226), demonstrates an ED50 below 1.0 ng/ml in Nb2-11 cell proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg—performance that supports receptor binding and competition assays, STAT5 phosphorylation studies, and cell-based signaling readouts such as cAMP induction. Endotoxin levels below 1.0 EU/μg, combined with purity greater than 98% by SDS-PAGE, satisfy the criteria typically required for in vivo pharmacology models investigating lactation, metabolic regulation, or immune modulation, as well as for use as a quantitative standard in immunoassays and antibody development campaigns. The authentic mature sequence also provides a suitable basis for structure-function studies probing receptor-binding determinants.