Mouse Receptor-interacting serine/threonine-protein kinase 3(RIPK3) ELISA kit

Code CSB-EL019737MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9QZL0
Lead Time 5-7 working days
Abbreviation RIPK3
Protein Biological Process 1 Immunity
Target Name receptor-interacting serine-threonine kinase 3
Alias RIP3, RIP-like protein kinase 3|receptor interacting protein 3
Species Mus musculus (Mouse)
Protein Biological Process 3 Host-virus interaction
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.72 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse RIPK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-96  
1:2 Average % 97  
Range % 92-102  
1:4 Average % 90  
Range % 87-97  
1:8 Average % 92  
Range % 85-96  
Recovery
The recovery of mouse RIPK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-98  
EDTA plasma (n=4) 98 95-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
3000 2.632 2.805 2.719 2.555  
1500 2.235 2.365 2.300 2.136  
750 1.819 1.903 1.861 1.697  
375 1.242 1.351 1.297 1.133  
187.5 0.660 0.714 0.687 0.523  
93.75 0.426 0.456 0.441 0.277  
46.88 0.272 0.250 0.261 0.097  
0 0.159 0.168 0.164    
References
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Function Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes
Subcellular Location Cytoplasm, cytosol, Cell membrane, Mitochondrion
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family
Tissue Specificity Expressed in embryo and in adult spleen, liver, testis, heart, brain and lung.
Database Links

KEGG: mmu:56532

STRING: 10090.ENSMUSP00000022830

UniGene: Mm.46612

Pathway TNF signaling pathway
Necroptosis
NOD-like receptor signaling pathway

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