Mouse Sonic hedgehog protein(SHH) ELISA kit

Instructions
Code CSB-EL021266MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name sonic hedgehog homolog (Drosophila)
Alternative Names HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, sonic hedgehog
Abbreviation SHH
Uniprot No. Q62226
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 28 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 83    
Range % 80-85    
1:2 Average % 91    
Range % 88-93    
1:4 Average % 102    
Range % 100-104    
1:8 Average % 86    
Range % 82-90    
Recovery
The recovery of mouse SHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 104 100-107    
EDTA plasma (n=4) 90 87-93    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1800 2.604 2.568 2.586 2.392    
900 2.037 2.004 2.021 1.827    
450 1.320 1.468 1.394 1.200    
225 0.933 0.945 0.939 0.745    
112.5 0.606 0.634 0.620 0.426    
56.25 0.382 0.391 0.387 0.193    
28 0.300 0.298 0.299 0.105    
0 0.196 0.192 0.194      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Target Data

Function Sonic hedgehog protein
Subcellular Location Sonic hedgehog protein N-product: Cell membrane, Lipid-anchor
Protein Families Hedgehog family
Tissue Specificity Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina.
Database Links

KEGG: mmu:20423

STRING: 10090.ENSMUSP00000002708

UniGene: Mm.57202

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