Mouse Transthyretin(TTR) ELISA kit

Code CSB-EL025270MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07309
Lead Time 5-7 working days
Abbreviation TTR
Protein Biological Process 1 Transport
Target Name transthyretin
Alias HsT2651, PALB, TBPA, prealbumin, amyloidosis type I|thyroxine-binding prealbumin
Species Mus musculus (Mouse)
Protein Biological Process 3 Transport
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc.
HGNC 12405
RGD 3916
MGI 98865
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %102
Range %96-108
1:2000Average %95
Range %91-99
1:4000Average %89
Range %85-94
1:8000Average %86
Range %82-92
Recovery
The recovery of mouse TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9590-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.285 2.184 2.235 2.061
1001.692 1.813 1.753 1.579
501.335 1.356 1.346 1.172
250.913 0.924 0.919 0.745
12.50.651 0.640 0.646 0.472
6.250.412 0.401 0.407 0.233
3.120.312 0.307 0.310 0.136
00.174 0.173 0.174  
References
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Function Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Subcellular Location Secreted
Protein Families Transthyretin family
Tissue Specificity Detected in plasma (at protein level). Detected in liver.
Database Links

KEGG: mmu:22139

STRING: 10090.ENSMUSP00000074783

UniGene: Mm.2108

Pathway Th17 cell differentiation
Thyroid hormone synthesis

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