Mouse Tyrosinase(TYR) ELISA kit

Instructions
Code CSB-EL025394MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name tyrosinase (oculocutaneous albinism IA)
Alternative Names Tyr ELISA Kit; Tyrosinase ELISA Kit; EC 1.14.18.1 ELISA Kit; Albino locus protein ELISA Kit; Monophenol monooxygenase ELISA Kit
Abbreviation TYR
Uniprot No. P11344
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TYR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %96
Range %93-99
1:2Average %88
Range %84-92
1:4Average %101
Range %98-104
1:8Average %86
Range %83-89
Recovery
The recovery of mouse TYR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9587-99
EDTA plasma (n=4)9895-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.156 2.169 2.163 2.038
10001.578 1.598 1.588 1.463
5001.031 1.004 1.018 0.893
2500.642 0.635 0.639 0.514
1250.410 0.435 0.423 0.298
62.50.316 0.326 0.321 0.196
31.250.201 0.216 0.209 0.084
00.124 0.126 0.125
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone
Gene References into Functions
  1. This study demonstrates the utility of CRISPR-Cas9 system in generating domain-specific in-frame deletions and helps gain further insights into structure-function of Tyr gene. PMID: 27224051
  2. These results suggest that the tyrosinase-induced metabolism of rhodendrol causes melanocyte toxicity. PMID: 25713930
  3. Data indicate that both ephedrannins A and B were competitive inhibitors of mushroom tyrosinase. PMID: 25857772
  4. STAP-2 colocalized with tyrosinase in lysosomes and protected tyrosinase from protein degradation in B16F10 melanoma cells. PMID: 26023234
  5. Docosahexaenoic acid inhibits melanogenesis in B16F10 cells in vitro through increased tyrosinase degradation. PMID: 24562306
  6. Studied upregulation by Kaliziri extract of tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells. PMID: 24884952
  7. The albino mutation of tyrosinase alters ocular angiogenic responsiveness. PMID: 23423728
  8. Tyrosinase is mutated in the albino dorsal lateral geniculate nucleus, arising from retinal ganglion cell misspecification together with potential perturbations of early activity patterns in the albino retina. PMID: 22492037
  9. activated by ascorbic acid in B16 melanoma via p38 MAPK signaling and upregulation of melanogenic regulatory factors PMID: 21667118
  10. Effect of zibuganshenfang on the expression and activity of tyrosinase on B16 cells PMID: 22256768
  11. the schisis phenotype caused by the Rs1 mutation is rescued by a Tyr mutation in the C57BL/6J genetic background, strongly suggesting that Tyr is the Mor1 gene. PMID: 20876567
  12. Data suggest that accumulation of tyrosinase in the endoplasmic reticulum of melanocytes leads to ER stress and activation of the unfolded protein response. PMID: 20444203
  13. Data show that interfering with p38 MAPK expression stimulates melanogenesis and tyrosinase family protein expression. PMID: 20053998
  14. role of P protein and tyrosinase in oculocutaneous albinism PMID: 12028586
  15. processing is controlled by pink-eyed dilution protein PMID: 12058062
  16. identified the tyrosinase gene (Tyr) as a modifier of the drainage structure phenotype in developmental glaucoma PMID: 12624268
  17. Tyrosinase maturation and oligomerization in the endoplasmic reticulum require a melanocyte-specific factor PMID: 12724309
  18. investigation of the boundary activity of locus control regions in transgenic animals and cells PMID: 14576318
  19. tyrosinase has a role in fatty acid regulation of pigmentation PMID: 14739285
  20. Interactions between N-linked glycans and lectin chaperones help to minimize tyrosinase aggregation and also target non-native substrates for retro-translocation and subsequent degradation PMID: 15161941
  21. Tyrosinase is a subject to post-Golgi quality control. PMID: 15760341
  22. tyrosinase has roles in the development of the visual system, as shown in a transgenic mouse model [review] PMID: 16146766
  23. tyrosinase and related proteins as physiological substrates of presenilins and link gamma-secretase activity with intracellular protein transport PMID: 16384915
  24. These results therefore suggest that proteasomes, particularly p27 subunit, are directly involved in the regulation of melanin biosynthesis in mouse melanoma cells. PMID: 16879986
  25. a 186 kb BAC containing the tyrosinase gene provides transgene expression in both RPE and melanocytes indicating the presence of regulatory sequences required for expression in the RPE PMID: 17196956
  26. Gallic acid effectively suppressed Tyr activity. PMID: 17541153
  27. The inhibitory effect of 4-hydroxybenzyl alcohol on melanogenesis is due to the direct inhibition of melanosomal Tyr activity, rather than to the suppression of Tyr gene. PMID: 17541167
  28. analysis of the mammalian tyrosinase active site with loss of function mutations PMID: 17850513
  29. these data demonstrate that Tyrp1 interacts directly with Tyrosinase in vivo, which may regulate the stability and trafficking of melanogenic enzymes and thus pigment synthesis. PMID: 18042623
  30. Results describe the molecular detection of circulating melanoma cells by reverse transcription-polymerase chain reaction (RT-PCR) in human blood samples and in a xenograft mouse model. PMID: 18425346
  31. the pigment-cell-specific cuproenzyme tyrosinase acquires copper only transiently and inefficiently within the trans-Golgi network of mouse melanocytes PMID: 18650808
  32. tyrosinase as a beta1,6 branch containing glycoprotein: substrate of GnT-V PMID: 18655794
  33. Data suggest that the tyrosinase promoter may be transiently active around E10 in subsets of precursor cells in the neural crest and intestines. PMID: 19220779

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Involvement in disease Defects in Tyr result in various forms of albinism. Himalayan strain tyrosinase is temperature-sensitive.
Subcellular Location Melanosome membrane, Single-pass type I membrane protein, Melanosome
Protein Families Tyrosinase family
Database Links

KEGG: mmu:22173

STRING: 10090.ENSMUSP00000004770

UniGene: Mm.238127

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