Recombinant Mouse Tyrosinase(Tyr)

Code CSB-CF025394MO
Size Pls inquire
Source in vitro E.coli expression system
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Names Tyr
Uniprot No. P11344
Alternative Names Tyr; Tyrosinase; EC 1.14.18.1; Albino locus protein; Monophenol monooxygenase
Species Mus musculus (Mouse)
Expression Region 19-533
Target Protein Sequence HFPRACASSKNLLAKECCPPWMGDGSPCGQLSGRGSCQDILLSSAPSGPQFPFKGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFGGPNCTEKRVLIRRNIFDLSVSEKNKFFSYLTLAKHTISSVYVIPTGTYGQMNNGSTPMFNDINIYDLFVWMHYYVSRDTLLGGSEIWRDIDFAHEAPGFLPWHRLFLLLWEQEIRELTGDENFTVPYWDWRDAENCDICTDEYLGGRHPENPNLLSPASFFSSWQIICSRSEEYNSHQVLCDGTPEGPLLRNPGNHDKAKTPRLPSSADVEFCLSLTQYESGSMDRTANFSFRNTLEGFASPLTGIADPSQSSMHNALHIFMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLLEVYPEANAPIGHNRDSYMVPFIPLYRNGDFFITSKDLGYDYSYLQESDPGFYRNYIEPYLEQASRIWPWLLGAALVGAVIAAALSGLSSRLCLQKKKKKKQPQEERQPLLMDKDDYHSLLYQSHL
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Applications : qPCR analysis

Review: The TYRP2 and Tyr-specific CD8+ T cells and serum anti-TYRP2 and Tyr antibodies were upregulated in AA mice. mRNA (Tyrp2 and Tyr) expressions in the skin analyzed by qPCR.

By Anonymous

Target Background

Function
(From Uniprot)
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone
Gene References into Functions
  1. This study demonstrates the utility of CRISPR-Cas9 system in generating domain-specific in-frame deletions and helps gain further insights into structure-function of Tyr gene. PMID: 27224051
  2. These results suggest that the tyrosinase-induced metabolism of rhodendrol causes melanocyte toxicity. PMID: 25713930
  3. Data indicate that both ephedrannins A and B were competitive inhibitors of mushroom tyrosinase. PMID: 25857772
  4. STAP-2 colocalized with tyrosinase in lysosomes and protected tyrosinase from protein degradation in B16F10 melanoma cells. PMID: 26023234
  5. Docosahexaenoic acid inhibits melanogenesis in B16F10 cells in vitro through increased tyrosinase degradation. PMID: 24562306
  6. Studied upregulation by Kaliziri extract of tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells. PMID: 24884952
  7. The albino mutation of tyrosinase alters ocular angiogenic responsiveness. PMID: 23423728
  8. Tyrosinase is mutated in the albino dorsal lateral geniculate nucleus, arising from retinal ganglion cell misspecification together with potential perturbations of early activity patterns in the albino retina. PMID: 22492037
  9. activated by ascorbic acid in B16 melanoma via p38 MAPK signaling and upregulation of melanogenic regulatory factors PMID: 21667118
  10. Effect of zibuganshenfang on the expression and activity of tyrosinase on B16 cells PMID: 22256768
  11. the schisis phenotype caused by the Rs1 mutation is rescued by a Tyr mutation in the C57BL/6J genetic background, strongly suggesting that Tyr is the Mor1 gene. PMID: 20876567
  12. Data suggest that accumulation of tyrosinase in the endoplasmic reticulum of melanocytes leads to ER stress and activation of the unfolded protein response. PMID: 20444203
  13. Data show that interfering with p38 MAPK expression stimulates melanogenesis and tyrosinase family protein expression. PMID: 20053998
  14. role of P protein and tyrosinase in oculocutaneous albinism PMID: 12028586
  15. processing is controlled by pink-eyed dilution protein PMID: 12058062
  16. identified the tyrosinase gene (Tyr) as a modifier of the drainage structure phenotype in developmental glaucoma PMID: 12624268
  17. Tyrosinase maturation and oligomerization in the endoplasmic reticulum require a melanocyte-specific factor PMID: 12724309
  18. investigation of the boundary activity of locus control regions in transgenic animals and cells PMID: 14576318
  19. tyrosinase has a role in fatty acid regulation of pigmentation PMID: 14739285
  20. Interactions between N-linked glycans and lectin chaperones help to minimize tyrosinase aggregation and also target non-native substrates for retro-translocation and subsequent degradation PMID: 15161941
  21. Tyrosinase is a subject to post-Golgi quality control. PMID: 15760341
  22. tyrosinase has roles in the development of the visual system, as shown in a transgenic mouse model [review] PMID: 16146766
  23. tyrosinase and related proteins as physiological substrates of presenilins and link gamma-secretase activity with intracellular protein transport PMID: 16384915
  24. These results therefore suggest that proteasomes, particularly p27 subunit, are directly involved in the regulation of melanin biosynthesis in mouse melanoma cells. PMID: 16879986
  25. a 186 kb BAC containing the tyrosinase gene provides transgene expression in both RPE and melanocytes indicating the presence of regulatory sequences required for expression in the RPE PMID: 17196956
  26. Gallic acid effectively suppressed Tyr activity. PMID: 17541153
  27. The inhibitory effect of 4-hydroxybenzyl alcohol on melanogenesis is due to the direct inhibition of melanosomal Tyr activity, rather than to the suppression of Tyr gene. PMID: 17541167
  28. analysis of the mammalian tyrosinase active site with loss of function mutations PMID: 17850513
  29. these data demonstrate that Tyrp1 interacts directly with Tyrosinase in vivo, which may regulate the stability and trafficking of melanogenic enzymes and thus pigment synthesis. PMID: 18042623
  30. Results describe the molecular detection of circulating melanoma cells by reverse transcription-polymerase chain reaction (RT-PCR) in human blood samples and in a xenograft mouse model. PMID: 18425346
  31. the pigment-cell-specific cuproenzyme tyrosinase acquires copper only transiently and inefficiently within the trans-Golgi network of mouse melanocytes PMID: 18650808
  32. tyrosinase as a beta1,6 branch containing glycoprotein: substrate of GnT-V PMID: 18655794
  33. Data suggest that the tyrosinase promoter may be transiently active around E10 in subsets of precursor cells in the neural crest and intestines. PMID: 19220779

Show More

Hide All

Involvement in disease Defects in Tyr result in various forms of albinism. Himalayan strain tyrosinase is temperature-sensitive.
Subcellular Location Melanosome membrane, Single-pass type I membrane protein, Melanosome
Protein Families Tyrosinase family
Database Links

KEGG: mmu:22173

STRING: 10090.ENSMUSP00000004770

UniGene: Mm.238127

  Tel: 301-363-4651
  Email: [email protected]
  Distributors Worldwide

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1