Mouse carcinoembryonic antign,CEA ELISA Kit

Code CSB-E13925m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q3UKK2
Lead Time 3-5 working days
Abbreviation CEACAM5
Target Name carcinoembryonic antigen-related cell adhesion molecule 5
Alias CD66e, CEA, DKFZp781M2392, OTTHUMP00000199034
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-93
1:2Average %98
Range %95-100
1:4Average %102
Range %97-107
1:8Average %94
Range %91-96
Recovery
The recovery of mouse CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9791-105
EDTA plasma (n=4)9390-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.522 2.635 2.579 2.402
20001.656 1.764 1.710 1.533
10001.047 1.095 1.071 0.894
5000.637 0.604 0.621 0.444
2500.366 0.390 0.378 0.201
1250.275 0.281 0.278 0.101
62.50.212 0.230 0.221 0.044
00.171 0.183 0.177
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Function Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling.
Protein Families Immunoglobulin superfamily, CEA family
Database Links

KEGG: mmu:73250

UniGene: Mm.244138

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