Mouse carcinoembryonic antign,CEA ELISA Kit

Code CSB-E13925m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
carcinoembryonic antigen-related cell adhesion molecule 5
Alternative Names
Ceacam5 ELISA Kit; Psg30Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; Pregnancy-specific glycoprotein 30 ELISA Kit
Abbreviation
CEACAM5
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-93
1:2Average %98
Range %95-100
1:4Average %102
Range %97-107
1:8Average %94
Range %91-96
Recovery
The recovery of mouse CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9791-105
EDTA plasma (n=4)9390-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.522 2.635 2.579 2.402
20001.656 1.764 1.710 1.533
10001.047 1.095 1.071 0.894
5000.637 0.604 0.621 0.444
2500.366 0.390 0.378 0.201
1250.275 0.281 0.278 0.101
62.50.212 0.230 0.221 0.044
00.171 0.183 0.177
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CEACAM5 ELISA Kit was designed for the quantitative measurement of Mouse CEACAM5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Target Background

Function
(From Uniprot)
Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.
Gene References into Functions
  1. a new mechanistic insight into how the CEA IgV-like N domain participates in cellular events that can have a macroscopic impact in terms of cancer progression and metastasis. PMID: 24388361
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface.
Protein Families
Immunoglobulin superfamily, CEA family
Database Links

KEGG: mmu:73250

UniGene: Mm.244138

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