Mouse hypoxia-inducible factor 1α,HIF-1α ELISA Kit

Instructions
Code CSB-E08541m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Alternative Names HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, ARNT interacting protein|hypoxia-inducible factor 1 alpha isoform I.3|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha
Abbreviation HIF1A
Uniprot No. Q61221
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 0.625 pg/mL-40 pg/mL
Sensitivity 0.156 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 100  
Range % 95-107  
1:200 Average % 89  
Range % 83-98  
1:400 Average % 91  
Range % 87-95  
1:800 Average % 96  
Range % 90-101  
Recovery
The recovery of mouse HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-95  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
40 2.253 2.233 2.243 2.186  
20 1.346 1.275 1.311 1.254  
10 0.792 0.752 0.772 0.715  
5 0.468 0.491 0.480 0.423  
2.5 0.263 0.264 0.264 0.207  
1.25 0.222 0.240 0.231 0.174  
0.625 0.118 0.122 0.120 0.063  
0 0.055 0.058 0.057    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity).
Subcellular Location Cytoplasm, Nucleus, Nucleus speckle
Tissue Specificity Ubiquitous.
Database Links

KEGG: mmu:15251

STRING: 10090.ENSMUSP00000021530

UniGene: Mm.3879

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