Mouse inducible nitric oxide synthase,iNOS ELISA KIT

Code CSB-E08326m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P29477
Lead Time 3-5 working days
Abbreviation NOS2
Target Name nitric oxide synthase 2, inducible
Alias HEP-NOS, INOS, NOS, NOS2A, NOS, type II|nitric oxide synthase 2A|nitric oxide synthase 2A (inducible, hepatocytes)|nitric oxide synthase, macrophage
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 7.8 IU/mL-500 IU/mL
Sensitivity 1.95 IU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-102  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 102  
Range % 98-107  
1:8 Average % 96  
Range % 92-102  
The recovery of mouse iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-105  
EDTA plasma (n=4) 95 91-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
IU/ml. OD1 OD2 Average Corrected  
500 2.599 2.581 2.590 2.403  
250 2.087 2.096 2.092 1.905  
125 1.341 1.369 1.355 1.168  
62.5 0.760 0.798 0.779 0.592  
31.2 0.499 0.480 0.490 0.303  
15.6 0.344 0.368 0.356 0.169  
7.8 0.282 0.293 0.288 0.101  
0 0.186 0.188 0.187    
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Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body
Protein Families NOS family
Tissue Specificity Macrophages.
Database Links

KEGG: mmu:18126

STRING: 10090.ENSMUSP00000018610

UniGene: Mm.2893

Pathway Calcium signaling pathway
HIF-1 signaling pathway
Apelin signaling pathway

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