Mouse interferon alpha-2(IFNA2) ELISA kit

Code CSB-EL011038MO
See More Details 24T ELISA kits trial application
Product Type ELISA kit
Size 96T,5×96T,10×96T
Uniprot No. P01573
Lead Time 3-5 working days
Abbreviation IFNA2
Target Name interferon alpha-2(IFNA2)
Alias IFN-alpha-2 Interferon alpha-A Short name= LeIF A
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 9.38 pg/mL-600 pg/mL
Sensitivity 2.35 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 5423
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-94
1:2 Average % 102
Range % 98-107
1:4 Average % 84
Range % 80-88
1:8 Average % 95
Range % 95-99
The recovery of mouse IFNA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 89-98
EDTA plasma (n=4) 98 94-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
600 2.651 2.536 2.594 2.440
300 2.049 1.928 1.989 1.835
150 1.376 1.315 1.346 1.192
75 0.892 0.952 0.922 0.768
37.5 0.568 0.529 0.549 0.395
18.75 0.374 0.364 0.369 0.215
9.4 0.237 0.241 0.239 0.085
0 0.150 0.157 0.154  
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Function Produced by macrophages, IFN-alpha have antiviral activities.
Subcellular Location Secreted
Protein Families Alpha/beta interferon family
Database Links

STRING: 10090.ENSMUSP00000100778

UniGene: Mm.14091

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway
Natural killer cell mediated cytotoxicity
NOD-like receptor signaling pathway
Toll-like receptor signaling pathway

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