Mouse interferon alpha-2(IFNA2) ELISA kit

Code CSB-EL011038MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name interferon alpha-2(IFNA2)
Alternative Names Ifna2Interferon alpha-2 ELISA Kit; IFN-alpha-2 ELISA Kit
Abbreviation IFNA2
Uniprot No. P01573
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 9.38 pg/mL-600 pg/mL
Sensitivity 2.35 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-94
1:2 Average % 102
Range % 98-107
1:4 Average % 84
Range % 80-88
1:8 Average % 95
Range % 95-99
The recovery of mouse IFNA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 89-98
EDTA plasma (n=4) 98 94-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
600 2.651 2.536 2.594 2.440
300 2.049 1.928 1.989 1.835
150 1.376 1.315 1.346 1.192
75 0.892 0.952 0.922 0.768
37.5 0.568 0.529 0.549 0.395
18.75 0.374 0.364 0.369 0.215
9.4 0.237 0.241 0.239 0.085
0 0.150 0.157 0.154  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

(From Uniprot)
Produced by macrophages, IFN-alpha have antiviral activities.
Subcellular Location Secreted.
Protein Families Alpha/beta interferon family
Database Links

STRING: 10090.ENSMUSP00000100778

UniGene: Mm.14091


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1