Mouse matrix metalloproteinase 10,MMP-10 ELISA kit

Code CSB-E07415m
Size 96T,5×96T,10×96T
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Product Details

Target Name
matrix metallopeptidase 10 (stromelysin 2)
Alternative Names
Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transin-2 ELISA Kit
Abbreviation
MMP10
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 101-114
1:2 Average % 91
Range % 86-95
1:4 Average % 98
Range % 94-102
1:8 Average % 87
Range % 82-93
Recovery
The recovery of mouse MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 88-96
EDTA plasma (n=4) 84 80-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.517 2.401 2.459 2.246
500 1.791 1.736 1.764 1.551
250 1.105 1.058 1.082 0.869
125 0.683 0.662 0.673 0.460
62.5 0.412 0.409 0.411 0.198
31.25 0.329 0.340 0.335 0.122
15.6 0.268 0.273 0.271 0.058
0 0.210 0.215 0.213  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse MMP10 ELISA Kit was designed for the quantitative measurement of Mouse MMP10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Gene References into Functions
  1. These results indicate that MMP10 serves a beneficial role in response to acute infection by moderating the proinflammatory response of resident and infiltrating macrophages. PMID: 27316687
  2. MMP-10 facilitates the clearance of multiwalled carbon nanotubesand moderates the pro-inflammatory response of exposed alveolar and infiltrated macrophages. PMID: 28223796
  3. crosstalk between MMP10 and the CXCR4/SDF1 axis contributes to hepatocarcinogenesis PMID: 25808184
  4. Matrix metalloproteinase-10 expression is induced during hepatic injury and plays a fundamental role in liver tissue repair. PMID: 24119197
  5. our findings support a model in which MMP-10 activity modulates CXCR4/SDF1 signaling, which is essential for efficient skeletal muscle regeneration. PMID: 24548137
  6. Dissection of the matrix metalloproteinase 10 (MMP10) substrate degradome in fibroblast secretomes. PMID: 24281761
  7. Thus, our findings indicate that MMP-10 is critical for skeletal muscle maintenance and regeneration during injury and disease. PMID: 24123596
  8. MMP10 promotes macrophage movement PMID: 23691065
  9. Assessed MMP-10's role in a murine model of colonic tissue damage induced by dextran sulfate sodium(DSS) treatment, and conclude MMP10 is required for resolution of DSS-induced colonic damage, and in its absence, chronic inflammation and dysplasia occurs. PMID: 23044923
  10. Mmp10 is required for maintenance of a highly tumorigenic, cancer-initiating, metastatic stem-like cell population in lung cancer. PMID: 22545096
  11. MMP-10 was capable of enhancing tissue plasminogen activator-induced fibrinolysis via a thrombin-activatable fibrinolysis inhibitor inactivation-mediated mechanism. PMID: 22104553
  12. Data show that S100a9, Mmp10 and Ang2 were among the most downregulated genes, whereas Per3, Tef and Prdx6 were significantly upregulated in the DMH + Calcium group. PMID: 21857934
  13. These findings support the hypothesis that CHF1/Hey2 is an important regulator of MMP10 expression. PMID: 22079635
  14. Data demonstrate induction of MMP-10 by VEGF in HUVEC and support an angiogenic role for MMP-10 in response to VEGF stimulation in vitro and in vivo. PMID: 20432469
  15. Results suggest that MMP-10 may be important in the initial stages of squamous cell cancer progression and induced in the stroma relating to the general host-response reaction to skin cancer. PMID: 19601983
  16. Mice injected with lymphoma cells constitutively expressing MMP10 develop thymic lymphoma more rapidly than those injected with control lymphoma cells. PMID: 15356104
  17. a tightly regulated expression level of stromelysin-2 is required for limited matrix degradation at the wound site PMID: 15371548
  18. These data indicate that MMP-10 does not significantly contribute to adipose tissue development and associated angiogenesis in a mouse model of nutritionally induced obesity. PMID: 19732744

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Tissue Specificity
Expressed in small intestine. Weak levels in heart and lung.
Database Links
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