Code | CSB-E07440m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
|
Have Questions? | Leave a Message or Start an on-line Chat |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIL-2Rα/CD25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 85 | ||||
Range % | 80-90 | |||||
1:2 | Average % | 108 | ||||
Range % | 104-112 | |||||
1:4 | Average % | 89 | ||||
Range % | 85-93 | |||||
1:8 | Average % | 107 | ||||
Range % | 103-110 |
The recovery of mouse sIL-2Rα/CD25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 88 | 84-93 | ||||
EDTA plasma (n=4) | 105 | 101-109 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
1000 | 2.813 | 2.690 | 2.752 | 2.575 | |||
500 | 2.221 | 2.301 | 2.261 | 2.084 | |||
250 | 1.583 | 1.601 | 1.592 | 1.415 | |||
125 | 0.945 | 0.931 | 0.938 | 0.761 | |||
62.5 | 0.626 | 0.601 | 0.614 | 0.437 | |||
31.2 | 0.387 | 0.361 | 0.374 | 0.197 | |||
15.6 | 0.303 | 0.302 | 0.303 | 0.126 | |||
0 | 0.176 | 0.178 | 0.177 |
|
This Mouse IL2RA ELISA Kit was designed for the quantitative measurement of Mouse IL2RA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/ml - 1000 pg/ml and the sensitivity is 3.9 pg/ml.
There are currently no reviews for this product.