Thrombin-antithrombin complex (TAT) is a stable covalent complex formed when thrombin, a key serine protease in the coagulation cascade, binds irreversibly to its primary physiological inhibitor, antithrombin. This complex serves as a sensitive biomarker of thrombin generation and coagulation activation, reflecting the balance between procoagulant and anticoagulant mechanisms. TAT levels are clinically relevant in various thrombotic disorders, cardiovascular diseases, and conditions involving hemostatic dysfunction. This makes it a valuable research tool for studying coagulation pathways and monitoring therapeutic interventions.
The Mouse thrombin-antithrombin complex TAT ELISA Kit (CSB-E08433m) provides quantitative measurement of TAT in Mus musculus samples. This sandwich ELISA works with serum, plasma, and tissue homogenates. The detection range spans 15.6 pg/mL to 1000 pg/mL with sensitivity of 3.9 pg/mL. The assay needs 50-100 μL sample volume, detects at 450 nm wavelength, and takes 1-5 hours to complete.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research studying coagulation and hemostatic processes in mouse models. The kit allows quantification of thrombin-antithrombin complex levels in mouse plasma samples, supporting studies that examine multiple coagulation-related biomarkers simultaneously.
• Coagulation research: Measuring thrombin-antithrombin complex levels alongside other hemostatic markers including fibrinogen and D-dimer in mouse plasma samples
• Inflammatory response studies: Studying coagulation parameters in conjunction with pro-inflammatory cytokines in cellular and plasma-based experimental systems
• Biomarker profiling: Multi-analyte analysis of coagulation cascade components in mouse models following surgical interventions or experimental treatments
• Hemostatic pathway analysis: Measuring thrombin-antithrombin complex formation as part of comprehensive coagulation status monitoring in research applications
