Mouse thrombin-antithrombin complex,TAT ELISA Kit

Code CSB-E08433m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name thrombin-antithrombin complex,TAT
Alternative Names N/A
Abbreviation TAT
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10 Average % 87  
Range % 82-93  
1:20 Average % 95  
Range % 90-101  
1:40 Average % 105  
Range % 96-112  
1:80 Average % 93  
Range % 87-98  
The recovery of mouse TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 93-100  
EDTA plasma (n=4) 90 85-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.658 2.456 2.557 2.374  
500 2.214 2.113 2.164 1.981  
250 1.503 1.522 1.513 1.330  
125 0.855 0.904 0.880 0.697  
62.5 0.542 0.563 0.553 0.370  
31.2 0.413 0.435 0.424 0.241  
15.6 0.291 0.302 0.297 0.114  
0 0.188 0.178 0.183    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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