Thrombin-antithrombin complex (TAT) serves as a biomarker for coagulation activation and thrombotic processes. This complex forms when thrombin, the key enzyme in blood coagulation, binds irreversibly to its primary physiological inhibitor, antithrombin. TAT levels reflect the balance between coagulation activation and inhibition, making it a valuable indicator for monitoring hemostatic disorders, thrombosis risk evaluation, and anticoagulant therapy effectiveness in research applications.
The Rat thrombin-antithrombin complex TAT ELISA Kit (CSB-E08432r) is designed for quantitative measurement of TAT in Rattus norvegicus samples. This sandwich ELISA accommodates serum, plasma, cell culture supernates, tissue homogenates, and cell lysates with a detection range of 31.25 pg/mL to 2000 pg/mL and sensitivity of 7.8 pg/mL. The assay requires 50-100 μL sample volume, operates with detection at 450 nm wavelength, and can be completed within 1-5 hours.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research examining coagulation and thrombosis-related processes across different experimental models. Studies have applied the kit to measure thrombin-antithrombin complex levels in various sample types including cell culture supernatants, tissue homogenates, and plasma samples. The applications span from cellular studies examining coagulation marker expression to animal model studies of hypercoagulability and thrombotic states.
• Coagulation research: Evaluation of thrombin-antithrombin complex levels alongside other hemostatic markers in studies examining blood coagulation processes and anticoagulant mechanisms
• Cerebrovascular studies: Measurement of coagulation markers in brain tissue homogenates from animal models studying ischemia-reperfusion injury and associated thrombotic responses
• Hypercoagulability evaluation: Analysis of plasma samples to evaluate markers of enhanced coagulation activity and thrombotic risk in experimental settings
• Cell culture applications: Quantification of coagulation-related proteins in cell supernatants to study cellular responses and protein expression under various experimental conditions
