Rat thrombin-antithrombin complex,TAT ELISA Kit

Code CSB-E08432r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat TAT ELISA Kit was designed for the quantitative measurement of Rat TAT protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

Target Name thrombin-antithrombin complex,TAT
Alternative Names N/A
Abbreviation TAT
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 97  
Range % 91-105  
1:10 Average % 103  
Range % 97-109  
1:20 Average % 95  
Range % 90-98  
1:40 Average % 96  
Range % 90-102  
Recovery
The recovery of rat TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 82-94  
EDTA plasma (n=4) 94 87-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.504 2.385 2.445 2.324  
1000 1.712 1.633 1.673 1.552  
500 1.010 1.001 1.006 0.885  
250 0.606 0.625 0.616 0.495  
125 0.387 0.396 0.392 0.271  
62.5 0.289 0.295 0.292 0.171  
31.25 0.193 0.196 0.195 0.074  
0 0.120 0.121 0.121    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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