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Pig thrombin-antithrombin complex,TAT ELISA Kit

Protocol MSDS
Code CSB-E13995p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
thrombin-antithrombin complex,TAT
Abbreviation
TAT
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %90
Range %84-100
1:2Average %97
Range %91-105
1:4Average %95
Range %90-108
1:8Average %95
Range %82-105
Recovery
The recovery of pig TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9385-102
EDTA plasma (n=4)9892-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.178 2.028 2.103 1.897
20001.582 1.560 1.571 1.365
10000.980 0.949 0.965 0.759
5000.558 0.565 0.562 0.356
2500.370 0.347 0.359 0.153
1250.296 0.302 0.299 0.093
62.50.265 0.275 0.270 0.064
00.200 0.212 0.206 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig TAT ELISA Kit was designed for the quantitative measurement of Pig TAT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Customer Reviews and Q&A

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■ Q&A
Q:

We are considering purchasing this product CSB-E13995p and I was reviewing the online documentation. The protocol specifies drawing blood samples using Li-heparin or K-EDTA as an anticoagulant. My laboratory has always used Na-citrate for ex-vivo anticoagulation when we are interested in measuring indices of coagulation like TAT. Has this variable been tested by CusaBio and is there a reason for what is put in the documentation?

A:

For the detection of plasma, usually we use EDTA and heparin as anticoagulants. The Na-citrate mentioned by you has little effect in theory. Pls follow the actual test results.

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