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| Code | CSB-E16311m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| The recovery of mouse TL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-93 | ||||
| EDTA plasma (n=4) | 94 | 87-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E16311m is a sandwich ELISA kit developed to measure levels of mouse tumor necrosis factor-like ligand 1 (TL1) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of TL1 bound in the initial step.
TL1, encoded by the gene TNFSF15, is a transmembrane protein produced by a variety of cell lines, including endothelial cells, macrophages, dendritic cells (DCs), and T cells. It plays a key role in the regulation and connection between innate immunity and adaptive immunity of mucosal inflammation and thus is implicated in inflammatory bowel disease. It is expressed by immune and endothelial cells in response to numerous pro-inflammatory stimuli. Through its receptor DR3/TNFRSF25, which is expressed primarily on lymphocytes, TL1 stimulates T cell activation, proliferation, and generation of a broad array of cytokines.
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