Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit

Instructions
Code CSB-E16311m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tumor necrosis factor (ligand) superfamily, member 15
Alternative Names Tnfsf15 ELISA Kit; Tl1 ELISA Kit; Vegi ELISA Kit; Tumor necrosis factor ligand superfamily member 15 ELISA Kit; TNF ligand-related molecule 1 ELISA Kit; Vascular endothelial cell growth inhibitor ELISA Kit
Abbreviation TNFSF15
Uniprot No. Q5UBV8
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-102  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 95  
Range % 90-100  
1:8 Average % 104  
Range % 100-108  
Recovery
The recovery of mouse TL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-93  
EDTA plasma (n=4) 94 87-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.871 2.641 2.756 2.601  
2000 2.086 2.067 2.077 1.922  
1000 1.348 1.325 1.337 1.182  
500 0.811 0.826 0.819 0.664  
250 0.505 0.544 0.525 0.370  
125 0.293 0.299 0.296 0.141  
62.5 0.219 0.235 0.227 0.072  
0 0.154 0.156 0.155    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis (By similarity). Promotes splenocyte alloactivation.
Gene References into Functions
  1. TL1A blocking ameliorates intestinal fibrosis in the T cell transfer model of chronic colitis PMID: 29254800
  2. ATF3 protects against LPS-induced acute lung injury by inhibiting TL1A expression. PMID: 28177121
  3. this study shows that TL1A-/- mice are more susceptible to dextran sodium sulfate colitis PMID: 27233964
  4. These data demonstrated a direct role for TL1A-DR3 signaling in tissue fibrosis and that modulation of TL1A-DR3 signaling could inhibit gut fibrosis. PMID: 24850426
  5. TL1A deficiency impacts on the gut microbial composition and the mucosal immune system, especially the intraepithelial TCRgammadelta(+) T-cell subset, and that TL1A is involved in the establishment of adipose tissue. PMID: 25404161
  6. Our data demonstrate a key role for TL1A in promoting ILC2s at mucosal barriers. PMID: 24220298
  7. delivers survival signals to plasma cells and promotes pathogenic Ab production in collagen arthritis PMID: 24140642
  8. TNFSF15 inhibits vasculogenesis by regulating relative levels of membrane-bound and soluble isoforms of VEGF receptor 1. PMID: 23918400
  9. These results establish a novel link between tumor necrosis factor (TNF)-family cytokine TL1A (TNFSF15) and interleukin 13 (IL-13) responses that results in small intestinal inflammation. PMID: 20980995
  10. role of TL1A in mucosal T cells and antigen presenting cells function and showed that up-regulation of TL1A expression can promote mucosal inflammation and gut fibrosis. PMID: 21264313
  11. findings suggest that the transcription factor NF-kappaB plays an important role in the regulation of VEGI expression PMID: 15702971
  12. neonatal CD4+ CD3- cells up-regulate both CD30L and OX40L after adoptive transfer into an adult recipient PMID: 16920944
  13. VEGI may have a key role in both the termination of angiogenesis and facilitation of dendritic cell maturation, which is the initial step in immune responses toward inflammatory stimuli. PMID: 17785811
  14. TL1A may contribute to renal inflammation and injury through DR3-mediated activation of NF-kappaB and caspase-3 PMID: 18287561
  15. Results reveal that TL1A is an important modulator in the development of chronic mucosal inflammation by enhancing T(H)1 and T(H)17 effector functions. PMID: 18598698

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Subcellular Location Membrane, Single-pass type II membrane protein
Protein Families Tumor necrosis factor family
Database Links

KEGG: mmu:326623

STRING: 10090.ENSMUSP00000050144

UniGene: Mm.208152

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