Mouse visfatin ELISA Kit

Instructions
Code CSB-E08942m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name nicotinamide phosphoribosyltransferase
Alternative Names Nampt ELISA Kit; Pbef1 ELISA Kit; Nicotinamide phosphoribosyltransferase ELISA Kit; NAmPRTase ELISA Kit; Nampt ELISA Kit; EC 2.4.2.12 ELISA Kit; Pre-B-cell colony-enhancing factor 1 homolog ELISA Kit; PBEF ELISA Kit; Visfatin ELISA Kit
Abbreviation NAMPT
Uniprot No. Q99KQ4
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse visfatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-95
1:2Average %98
Range %94-100
1:4Average %102
Range %94-107
1:8Average %94
Range %87-98
Recovery
The recovery of mouse visfatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-102
EDTA plasma (n=4)9388-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.533 2.695 2.614 2.434
101.867 1.764 1.816 1.636
51.016 1.097 1.057 0.877
2.50.678 0.713 0.696 0.516
1.250.382 0.390 0.386 0.206
0.6250.276 0.270 0.273 0.093
0.3120.224 0.220 0.222 0.042
00.174 0.185 0.180
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma (By similarity). Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component
Gene References into Functions
  1. our study demonstrated that PBEF ameliorates ischemia-induced neuronal death through inhibiting caspase-dependent and independent apoptotic signaling pathways and suppressing mitochondrial damage and dysfunction. Our study provides novel insights into the mechanisms underlying the neuroprotective effect of PBEF, and helps to identify potential targets for ischemic stroke therapy. PMID: 27576732
  2. the NAMPT-mediated NAD(+) biosynthesis pathway is a potential therapeutic target for degenerative motor neuron diseases. PMID: 28854367
  3. observations demonstrate that NAMPT knockdown exerted antiatherogenic effects by promoting cholesterol efflux and macrophage RCT through the PPARalpha- LXRalpha- ABCA1/G1pathway in vitro and in vivo PMID: 27229177
  4. Study found that primary mouse embryonic fibroblast (MEF) cells undergo progressive decline of NAMPT and NAD(+) contents during serial passaging before becoming senescent; further found that constitutive Nampt over-expression increases SIRT1 activity, increases the expression of antioxidant genes, superoxide dismutase 2 and catalase and promotes resistance against oxidative stress. PMID: 29178516
  5. NAMPT-mediated NAD(+) biosynthesis in adipocytes regulates adipose tissue function and multi-organ insulin sensitivity in mice. PMID: 27498863
  6. NAMPT-mediated NAD(+) biosynthesis is essential for vision in mice. PMID: 27681422
  7. NAMPT is involved in key basic cellular functions. PMID: 28333140
  8. role of Nampt in regulating osteoblast differentiation in primary mouse bone marrow-derived mesenchymal stem cells (BM-MSCs); Nampt expression was progressively elevated during BM-MSCs osteogenic differentiation PMID: 28159473
  9. Synthesis and Degradation of Adenosine 5'-Tetraphosphate by Nicotinamide and Nicotinate Phosphoribosyltransferases PMID: 28416276
  10. NAMPT inhibited endothelial progenitor cell senescence through an SIRT1 AS lncRNA/miR-22/SIRT1 pathway PMID: 27569277
  11. results support a novel, important role for Nampt-mediated NAD(+) biosynthesis in long-term depression and in the function of GluN2B-containing NMDARs PMID: 26481044
  12. extracellular NAMPT can be actively released by melanoma cells in vitro. PMID: 26358657
  13. Visfatin plays an important role in glucose metabolism via the calcium mediated AMPK-p38 MAPK pathway. PMID: 26019302
  14. Deacetylation of intracellular NAMPT by the mammalian NAD(+)-dependent deacetylase SIRT1 predisposes the protein to secretion in adipocytes. PMID: 25921090
  15. Report retinal toxicity of NAMPT inhibitors. PMID: 25505128
  16. intracellular NAMPT-NAD(+)-SIRT1 cascade improves post-ischaemic neovascularization PMID: 25341895
  17. The Nampt activity of visfatin is involved in chondrocyte and osteoblast activation PMID: 24479481
  18. Visfatin levels increase after aortic valve replacement for aortic stenosis and may play a part in postoperative reverse remodelling. PMID: 24429804
  19. nicotinamide phosphoribosyltransferase increases NAD synthesis in muscle but is not sufficient to promote oxidative metabolism PMID: 25411251
  20. NAMPT is a key novel ARDS therapeutic target and candidate gene with genetic/epigenetic transcriptional regulation in response to excessive mechanical stress. PMID: 24821571
  21. Ischemic Injury Induces Nicotinamide Phosphoribosyltransferase Expression and Secretion from Neurons. PMID: 25005877
  22. Pharmacological inhibition of NAMPT activity mitigates inflammation in atherosclerotic plaques by reducing CXCL1-mediated activities on neutrophils. PMID: 24196571
  23. Oral lipid ingestion is a physiological regulator of systemic visfatin release. Fatty acids differentially regulate visfatin release in vitro. PMID: 24554513
  24. Fndings support NAMPT inhibition via FK-866 as a novel therapeutic agent for acute respiratory distress syndrome via enhanced apoptosis in inflammatory neutrophils. PMID: 24588101
  25. results indicate that JAZF1 might firstly upregulated the expression of PPARalpha and PPARbeta/delta, which in turn activated the transcription of visfatin PMID: 24930994
  26. Specific ablation of Nampt in adult neural stem cells recapitulates their functional defects during aging. PMID: 24811750
  27. miR-34a reduces NAD(+) levels and SIRT1 activity by targeting NAMPT, the rate-limiting enzyme for NAD(+) biosynthesis. PMID: 23834033
  28. forebrain excitatory neurons mainly use intracellular Nampt-mediated NAD(+) biosynthesis to mediate their survival and function. PMID: 24760840
  29. GLP-1 promotes the novel insulin-mimetic adipocytokine visfatin expression. PMID: 23670349
  30. These results suggested that diet-induced obesity could promote murine gastric cancer growth by upregulating the expression of the nampt, sirt1 and c-myc genes. PMID: 23970286
  31. melatonin suppresses visfatin-induced iNOS upregulation in macrophages by inhibiting the STAT3 and NF-kappaB pathways. PMID: 23869429
  32. Rev-erb alpha is a new intracellular regulator of glucagon secretion via AMPK/Nampt/Sirt1 pathway. PMID: 23936124
  33. Nicotinamide phosphoribosyltransferase/visfatin expression by inflammatory monocytes mediates arthritis pathogenesis. PMID: 23313810
  34. Osteogenic differentiation correlates with intracellular nicotinamide adenine dinucleotide (NAD) metabolism in which Nampt plays a regulatory role. PMID: 23537654
  35. Nampt inhibition appears as a new strategy to dampen CXCL2-induced neutrophil recruitment and thereby reduce neutrophil-mediated tissue injury in mice PMID: 22452634
  36. cardiomyocytes are capable of secreting Nampt during stress, and exogenous Nampt is a positive regulator of cardiac hypertrophy and adverse ventricular remodeling PMID: 23203961
  37. the proinflammatory actions of visfatin in chondrocytes involve regulation of IR signaling pathways, possibly through the control of Nampt enzymatic activity PMID: 22399297
  38. This study indicates that plasma visfatin levels are significantly higher in ST-elevation myocardial infarction patients. PMID: 22137121
  39. Visfatin administration during superovulation plays an important role in regulating oocyte quality and can improve oocyte quality and fertility of aged female mice. PMID: 22425197
  40. Knockdown of Nampt in the mouse mesenchymal cell line C3H10T1/2 cells resulted in decreased Sirt1 activity and enhanced adipogenesis. PMID: 21812028
  41. FoxOs control the Nampt gene expression and the NAD(+) signaling in the regulation of hepatic triglyceride homeostasis PMID: 21388966
  42. study shows that PBEF has a neuronal protective role in cerebral ischemia presumably through enhanced energy metabolism PMID: 20485294
  43. analysis of inhibition of nicotinamide phosphoribosyltransferase and how cellular bioenergetics reveals a mitochondrial insensitive NAD pool PMID: 20724478
  44. study examined the effect of visfatin on cellular stress responses and signalling pathways in skeletal muscle; findings provide novel insights for the unique pathophysiological role of visfatin in skeletal muscle PMID: 19898975
  45. activated, but not resting, T lymphocytes undergo massive NAD(+) depletion upon FK866-mediated Nampt inhibition PMID: 19936064
  46. Estriol, estradiol and progesterone exert a synergistic effect on visfatin gene expression. Visfatin may play a physiological role during pregnancy. PMID: 20004694
  47. Data show that visfatin exists in all the detected tissues, with the expression in spleen and adipose tissue higher than the others. PMID: 19500503
  48. The role of Nampt in regulating autophagy and potential mechanisms by which NAD(+) regulates autophagy in the heart, is discussed. PMID: 19855187
  49. Pre-B-cell colony-enhancing factor, whose expression is up-regulated in activated lymphocytes, is a nicotinamide phosphoribosyltransferase, a cytosolic enzyme involved in NAD biosynthesis. PMID: 12555668
  50. NAD biosynthesis mediated by Nampt regulates the function of Sir2alpha and plays an important role in controlling various biological events in mammals PMID: 15381699

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Subcellular Location Nucleus, Cytoplasm, Secreted
Protein Families NAPRTase family
Tissue Specificity Ubiquitously expressed in lymphoid and non-lymphoid tissues.
Database Links

KEGG: mmu:59027

STRING: 10090.ENSMUSP00000020886

UniGene: Mm.202727

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