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Pig Angiopoietin 1,ANG-1 ELISA Kit

Protocol
Code CSB-E08457p
Size 96T,5×96T,10×96T
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Product Details

Target Name
angiopoietin 1
Alternative Names
ANGPT1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit
Abbreviation
ANGPT1
Uniprot No.
Q9BDY8
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6 ng/mL-200 ng/mL
Sensitivity
6 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 95    
Range % 90-105    
1:2 Average % 95    
Range % 85-100    
1:4 Average % 89    
Range % 85-101    
1:8 Average % 90    
Range % 80-99    
Recovery
The recovery of pig ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 92-105    
EDTA plasma (n=4) 96 90-102    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
200 0.345 0.351 0.348      
100 0.478 0.486 0.482      
50 0.703 0.716 0.710      
17 1.068 1.103 1.086      
6 1.316 1.355 1.336      
0 1.925 1.968 1.947      
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Related Products

ANGPT1 Proteins

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Target Background

Function
(From Uniprot)
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
Gene References into Functions
  1. Angiopoietin 1 was predominantly present in the maturing glomeruli. PMID: 20598355
  2. The expression of ANGPT1 has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros. PMID: 20713984
  3. Attenuates H2O2-induced stress-activated protein kinase (SEK)1/JNK phosphorylation through the PI 3-kinase/Akt pathway and inhibits the apoptosis of vascular endothelial cells induced by oxidative stress. PMID: 16000309
  4. Estrogen-dependent control of Ang-1 expression plays an important role in stabilizing meningeal microvessel and maintaining healthy microvascular networks. PMID: 17496211
Subcellular Location
Secreted.
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