Product Details

Target Name

CD163 molecule

Alternative Names

CD163 ELISA Kit; M130Scavenger receptor cysteine-rich type 1 protein M130 ELISA Kit; CD antigen CD163) [Cleaved into: Soluble CD163 ELISA Kit; sCD163)] ELISA Kit

Abbreviation

CD163

Uniprot No.

Q2VL90

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

93.75 ng/mL-6000 ng/mL

Sensitivity

23.4 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig CD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	94
	Range %	91-97
1:4	Average %	103
	Range %	100-106
1:8	Average %	99
	Range %	95-103

Recovery

The recovery of pig CD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	89	86-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
6000	2.111	2.228	2.170	2.003
3000	1.365	1.455	1.410	1.243
1500	0.845	0.868	0.857	0.690
750	0.555	0.574	0.565	0.398
375	0.369	0.349	0.359	0.192
187.5	0.282	0.263	0.273	0.106
93.75	0.235	0.230	0.233	0.066
0	0.170	0.163	0.167

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig CD163 ELISA Kit was designed for the quantitative measurement of Pig CD163 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 ng/mL-6000 ng/mL and the sensitivity is 23.4 ng/mL.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 11 reviews

Submit a Review here

Sample type: Tissue homogenate

Sample species: Pig

Sample dilution: No dilution

Review: I used this kit to test tissues and sera, and the od values were all in normal units. The od of the standard curve was 1.856, 1.609, etc. The square of r was 0.997. There were also differences among each tissue, which was quite good

By Anonymous

Sample type: Tissue homogenate

Sample species: Pig

Sample dilution: No dilution

Review: I used this kit to test the protein content of various edible parts of pigs. All indicators were normal, with high accuracy and strong specificity. Overall, compared with similar elisa kits in the same industry, it brought me an excellent experimental experience and results

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

Review: Huamei Biological\'s ELISA KIT research and development experience is extensive, with over 4,000 types covering multiple fields and species. It features strict quality control and high stability, earning the trust of many scientific researchers.

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

Review: Huamei Biological\'s ELISA KIT research and development experience is rich, with a wide variety covering multiple fields and species. It features strict quality control and high stability, earning the trust of many scientific researchers. The experimental operation is simple and fast, and the experimental results are stable.

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

Review: Huamei Biological\'s ELISA KIT research and development experience is rich, with a wide variety of types covering multiple fields and species. It features strict quality control and high stability, earning the trust of many scientific researchers. The experimental results are stable and in line with the expectations of the experiment

By Anonymous

Sample type: Tissue homogenate

Sample species: Pig

Sample dilution: No dilution

Review: I used your company\'s reagent kit and obtained reliable data. The design of the reagent kit is reasonable, the process is simple and easy to operate, and the standard curve has reached 999. The data analysis results also meet expectations

By Anonymous

Sample type: Tissue homogenate

Sample species: Pig

Sample dilution: No dilution

Review: Using your company\'s cd163 kit, the specificity is quite good, the standard curve fitting degree is quite high, the r square is above 0.99, and the repeatability between each tissue is also good

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

Review: The experimental results are all in line with expectations. The measurement results are all within the range. The standard curves also meet the standards. The measurement results are relatively sensitive. The operation is simple and the waiting period is normal.

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

By Anonymous

Read more reviews >>

Target Background

Function
(From Uniprot)

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. May play a role in the process of infection of porcine monocytes/macrophages by African swine fever virus (ASFV). In case of porcine reproductive and respiratory syndrome virus (PRRSV), serves mediates virion attachment and plays a role in viral entry.; After shedding, the soluble form (sCD163) may play an anti-inflammatory role.

Gene References into Functions

Study investigated the association of three CD163 polymorphisms with host immune responses following PRRSV challenge and also analyzed the combined effects of the CD163 SNPs. The most significant associations were found with the c.2509G>C, c.2638A>G and c.3534C>T polymorphisms. PMID: 29285769
deletion of Exon 7 of the CD163 gene, encoding SRCR5 had no adverse effects in pigs and these animals showed complete resistance to viral infection by the positive-strand RNA PRRS virus PMID: 28231264
CD163 is not necessary for infection with African swine fever virus. PMID: 27898335
CD163 represents an additional candidate gene for resistance against porcine reproductive and respiratory syndrome. PMID: 26711047
Overexpression of ADAM17 induced downregulation of CD163 expression and a reduction in reproductive and respiratory syndrome virus infection. PMID: 24965453
Fusion protein of sialoadhesin and domains 5-9 of CD163 receptors blocked the respiratory syndrome virus infection. PMID: 24246307
GP4 is involved in interaction with cellular receptor CD163. PMID: 22537809
). These results provided fundamental evidence for CD163 and SN as two functional candidate genes affecting immunity in pigs. PMID: 21786159
Porcine reproductive and respiratory syndrome virus GP4 was found to co-localize with CD163 in the lipid rafts on the plasma membrane. PMID: 22222209
Our results suggest that the intracellular domain of CD163 may be associated with an important yet-unknown function during porcine reproductive and respiratory syndrome virus replication. PMID: 20496088
The newly established porcine alveolar macrophage cell lines were demonstrated to express robust levels of CD163 and to be fully permissive for both type 1 and 2 porcine reproductive and respiratory syndrome virus strains. PMID: 19900480
The carboxy-terminal 223 residues of the CD163 molecule are not required for interactions with either the GP2a or the GP4 protein, although these residues are required for conferring susceptibility to PRRSV infection. PMID: 19939927
CD163 is shown to be involved in PRRSV (porcine reproductive and respiratory syndrome virus) entry, probably during uncoating, and to interact with sialoadhesin in the process. PMID: 19008379
The expression level of CD163 correlates with the level of PRRSV infection. PMID: 19111584
Clear changes in the quantity of sialoadhesin(+) and CD163(+) macrophages in the placentas and organs of embryos/fetuses during gestation. PMID: 19410291

Hide All

Subcellular Location

[Soluble CD163]: Secreted.; Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in monocytes and macrophages. Detected only in one population of monocytes (CD163+) which is in advanced maturation stage.

Database Links

KEGG: ssc:397031

UniGene: Ssc.100404

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-EL004882PI
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
Have Questions?	Leave a Message or Start an on-line Chat

Pig Scavenger receptor cysteine-rich type 1 protein M130(CD163) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background