Pig Scavenger receptor cysteine-rich type 1 protein M130(CD163) ELISA kit

Code CSB-EL004882PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name CD163 molecule
Alternative Names CD163 ELISA Kit; M130Scavenger receptor cysteine-rich type 1 protein M130 ELISA Kit; CD antigen CD163) [Cleaved into: Soluble CD163 ELISA Kit; sCD163)] ELISA Kit
Abbreviation CD163
Uniprot No. Q2VL90
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 ng/mL-6000 ng/mL
Sensitivity 23.4 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of pig CD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-90
1:2 Average % 94
Range % 91-97
1:4 Average % 103
Range % 100-106
1:8 Average % 99
Range % 95-103
The recovery of pig CD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 92-98
EDTA plasma (n=4) 89 86-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
6000 2.111 2.228 2.170 2.003
3000 1.365 1.455 1.410 1.243
1500 0.845 0.868 0.857 0.690
750 0.555 0.574 0.565 0.398
375 0.369 0.349 0.359 0.192
187.5 0.282 0.263 0.273 0.106
93.75 0.235 0.230 0.233 0.066
0 0.170 0.163 0.167  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity). May play a role in the process of infection of porcine monocytes/macrophages by African swine fever virus (ASFV). In case of porcine reproductive and respiratory syndrome virus (PRRSV), serves mediates virion attachment and plays a role in viral entry.
Gene References into Functions
  1. Study investigated the association of three CD163 polymorphisms with host immune responses following PRRSV challenge and also analyzed the combined effects of the CD163 SNPs. The most significant associations were found with the c.2509G>C, c.2638A>G and c.3534C>T polymorphisms. PMID: 29285769
  2. deletion of Exon 7 of the CD163 gene, encoding SRCR5 had no adverse effects in pigs and these animals showed complete resistance to viral infection by the positive-strand RNA PRRS virus PMID: 28231264
  3. CD163 is not necessary for infection with African swine fever virus. PMID: 27898335
  4. CD163 represents an additional candidate gene for resistance against porcine reproductive and respiratory syndrome. PMID: 26711047
  5. Overexpression of ADAM17 induced downregulation of CD163 expression and a reduction in reproductive and respiratory syndrome virus infection. PMID: 24965453
  6. Fusion protein of sialoadhesin and domains 5-9 of CD163 receptors blocked the respiratory syndrome virus infection. PMID: 24246307
  7. GP4 is involved in interaction with cellular receptor CD163. PMID: 22537809
  8. ). These results provided fundamental evidence for CD163 and SN as two functional candidate genes affecting immunity in pigs. PMID: 21786159
  9. Porcine reproductive and respiratory syndrome virus GP4 was found to co-localize with CD163 in the lipid rafts on the plasma membrane. PMID: 22222209
  10. Our results suggest that the intracellular domain of CD163 may be associated with an important yet-unknown function during porcine reproductive and respiratory syndrome virus replication. PMID: 20496088
  11. The newly established porcine alveolar macrophage cell lines were demonstrated to express robust levels of CD163 and to be fully permissive for both type 1 and 2 porcine reproductive and respiratory syndrome virus strains. PMID: 19900480
  12. The carboxy-terminal 223 residues of the CD163 molecule are not required for interactions with either the GP2a or the GP4 protein, although these residues are required for conferring susceptibility to PRRSV infection. PMID: 19939927
  13. CD163 is shown to be involved in PRRSV (porcine reproductive and respiratory syndrome virus) entry, probably during uncoating, and to interact with sialoadhesin in the process. PMID: 19008379
  14. The expression level of CD163 correlates with the level of PRRSV infection. PMID: 19111584
  15. Clear changes in the quantity of sialoadhesin(+) and CD163(+) macrophages in the placentas and organs of embryos/fetuses during gestation. PMID: 19410291

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Subcellular Location Soluble CD163: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein
Tissue Specificity Expressed in monocytes and macrophages. Detected only in one population of monocytes (CD163+) which is in advanced maturation stage.
Database Links

KEGG: ssc:397031

UniGene: Ssc.100404


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