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Pig calprotectin (CALP) ELISA kit

Citations (4) Protocol
Code CSB-EQ013485PI
Size 96T,5×96T,10×96T
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Product Details

Target Name
Calprotectin
Abbreviation
CALP
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:100 Average % 96    
Range % 91-102    
1:200 Average % 85    
Range % 81-89    
1:400 Average % 103    
Range % 98-107    
1:800 Average % 87    
Range % 83-92    
Recovery
The recovery of pig CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 94 88-97    
EDTA plasma (n=4) 106 102-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
20 0.099 0.101 0.100      
10 0.160 0.168 0.164      
5 0.241 0.255 0.248      
2.5 0.365 0.373 0.369      
1.25 0.544 0.556 0.550      
0.625 0.713 0.755 0.734      
0.312 0.984 1.044 1.014      
0 1.765 1.834 1.800      
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Pig CALP ELISA Kit was designed for the quantitative measurement of Pig CALP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Citations

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