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| Code | CSB-EQ013485DO |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of canine CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 81-92 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 85-99 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 96-107 | ||||||
| The recovery of canine CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 89-98 | |||||
| EDTA plasma (n=4) | 102 | 97-106 | |||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Dog CALP ELISA Kit was designed for the quantitative measurement of Dog CALP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
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Would you provide the information:
1) antibody clonality _ are capture and detection antibodies monoclonal or polyclonal?
2) antibody's target immunogen _ to which immunogen have detection and capture antibodies of the kit been raised? was it of human or canine origin? was it a native or recombinant protein? was it a full length protein or a fragment?
3) proteins detected in the samples by the antibodies _ do the
antibodies of the kit detect only the S100A8/S100A9 heterocomplex or do they also detect S100A8 and S100A9 proteins separately (as monomers or homodimers)?
4) standard protein _ is the protein used as the analytical standard of the kit of canine or human origin? is it a native or recombinant protein? is it a full length protein or a fragment?
