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Canine calprotectin (CALP) ELISA kit

Citations (1) Protocol MSDS
Code CSB-EQ013485DO
Size 96T,5×96T,10×96T
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Product Details

Target Name
Calprotectin
Abbreviation
CALP
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
6.25 ng/mL-400 ng/mL
Sensitivity
1.56 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 86    
Range % 81-92    
1:2 Average % 102    
Range % 97-107    
1:4 Average % 96    
Range % 85-99    
1:8 Average % 103    
Range % 96-107    
Recovery
The recovery of canine CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 94 89-98    
EDTA plasma (n=4) 102 97-106    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
400 1.816 1.921 1.869 1.723    
200 1.448 1.512 1.480 1.334    
100 1.030 1.078 1.054 0.908    
50 0.758 0.733 0.746 0.600    
25 0.476 0.466 0.471 0.325    
12.5 0.348 0.332 0.340 0.194    
6.25 0.275 0.268 0.272 0.126    
0 0.145 0.146 0.146 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Dog CALP ELISA Kit was designed for the quantitative measurement of Dog CALP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

Citations

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Customer Reviews and Q&A

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■ Q&A
Q:

Would you provide the information:
1) antibody clonality _ are capture and detection antibodies monoclonal or polyclonal?
2) antibody's target immunogen _ to which immunogen have detection and capture antibodies of the kit been raised? was it of human or canine origin? was it a native or recombinant protein? was it a full length protein or a fragment?
3) proteins detected in the samples by the antibodies _ do the
antibodies of the kit detect only the S100A8/S100A9 heterocomplex or do they also detect S100A8 and S100A9 proteins separately (as monomers or homodimers)?
4) standard protein _ is the protein used as the analytical standard of the kit of canine or human origin? is it a native or recombinant protein? is it a full length protein or a fragment?

A:
Thanks for your inquiry!CSB-EQ013485DO detects CALP(Calprotectin is a complex of the mammalian proteins S100A8 and S100A9)
The kit uses 2 antibodies, the Coated anti. and Detect anti. are both Goat polyclonal antibody, which are designed for the information of the links
https://www.ncbi.nlm.nih.gov/protein/XP_013970901.1 and https://www.ncbi.nlm.nih.gov/protein/NP_001139616.1 separately.
The immunogen is 293 cell-derived recombinant protein.
The standard is the purified crude product of the natural sample,but so sorry the specific source information can not be disclosed.
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