Pig endothelin 1,ET-1 ELISA Kit

Instructions
Code CSB-E06806p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name endothelin 1
Alternative Names EDN1 ELISA Kit; Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
Abbreviation EDN1
Uniprot No. P09558
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %100
Range %94-105
1:2Average %97
Range %92-101
1:4Average %89
Range %85-93
1:8Average %95
Range %91-101
Recovery
The recovery of pig ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-94
EDTA plasma (n=4)8480-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.401 2.449 2.425 2.354
1001.689 1.661 1.675 1.604
500.925 0.912 0.919 0.848
250.491 0.459 0.475 0.404
12.50.302 0.286 0.294 0.223
6.250.221 0.213 0.217 0.146
3.120.158 0.154 0.156 0.085
00.068 0.074 0.071  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
Gene References into Functions
  1. In conclusion, endothelial vWF knockdown prevented angiotensin II-induced ET-1 upregulation through attenuation of NOX-mediated O2- production. PMID: 27443965
  2. sub-vasomotor concentration of ET-1 leads to vascular dysfunction by impairing endothelium-dependent NO-mediated dilation via p38 kinase-mediated production of superoxide from NADPH oxidase following ETA receptor activation PMID: 26211713
  3. ET1 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest. PMID: 25380860
  4. ET-1 contributes to formation of oedema during experimental sepsis by a novel mechanism involving increased HBP release from neutrophils. PMID: 24611481
  5. Endothelin-1 expression is upregulated following therapeutic hypothermia after cardiac arrest. PMID: 23717659
  6. Our experiment with isolated porcine ophthalmic ciliary arteries revealed a refractoriness phase to ET after an acute stimulation with ET. This refractoriness was transient and disappeared after 4 h. PMID: 23327561
  7. ET-1 produces contraction of the urinary bladder neck muscles via muscular ET(A) receptors coupled to extracellular Ca(2+) entry via VOC (L-type) and non-VOC channels. PMID: 21953705
  8. urinary biomarker to determine kidney graft injury PMID: 22221604
  9. Endothelin-1 elevation is not only a conserved phenomenon in a pig traumatic brain injury (TBI) model, but it is a likely target for understanding the observed enhanced vascular response to TBI. PMID: 21801588
  10. interleukin-6, endothelin ET-1, and apoptotic Bak and Bcl-XL genes have roles in small bowel transplantation, in a swine model of ischemia and reperfusion injury PMID: 21884347
  11. ET-1 elicits a different pattern of Src family kinase (SFK) activation and might trigger a different pattern of SFK activation from that caused by ATP and UTP. PMID: 21792912
  12. Compared with the untreated group, the levels of serum ET-1 after acute myocardial infarction and reperfusion were significantly decreased in the Xuefu Zhuyu-treated group. PMID: 18405606
  13. study suggests a possible role for endothelin-1(resulting from the actions of endothelin-converting enzyme-1) acting via endothelin receptor A in the control of luteolytic sensitivity in the pig PMID: 19783117
  14. vasoconstrictor influence of endogenous as well as exogenous ET on coronary circulation in vivo is reduced in myocardial infarct PMID: 15458948
  15. Data show the presence of a continuative and differentially regulated stimulating mechanism of ET-1 expression during pig endotoxaemia as well as a fundamental role of ET-1 system in the cardiovascular and respiratory derangement. PMID: 16043243
  16. These data support a linkage between the proinflammatory cytokine IL-1beta and vascular dysfunction within the intestinal circulation, mediated, at least in part, by the ET system. PMID: 16782700
  17. extracellular ET-1 regulates the abundance of ET-1 mRNA in PAECs, in an ETB receptor-dependent manner, by modulating both mRNA stability and transcription via mechanisms involving receptor endocytosis and both ERK and p38 MAPK pathways PMID: 18278064
  18. Cyclosporine-A stimulates angiotensin I/II secretion by kidney glomeruli. PMID: 18331442
  19. Left ventricular unloading with intra-aortic counter pulsation prior to reperfusion reduces myocardial release of endothelin-1 and decreases infarction size in a porcine ischemia-reperfusion model. PMID: 18814226
  20. The beneficial effect of carvedilol on myocardial no-reflow could be due to its effect on ET-1 via the activation of the K(ATP) channel. PMID: 18840580
  21. is not predictive of ventricular fibrillation during acute myocardial ischemia PMID: 18844577
  22. Porcine ET-1 rapidly activates the prosurvival phosphatidylinositol 3'-OH kinase (PI3K)/AKT signaling pathway in normal and fibrotic human lung fibroblasts. PMID: 19188658
  23. Report novel pathway involving endothelin-1 and reactive oxygen species in coronary vasospasm with endothelial dysfunction. PMID: 19623039

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Subcellular Location Secreted.
Protein Families Endothelin/sarafotoxin family
Database Links

KEGG: ssc:396915

STRING: 9823.ENSSSCP00000001121

UniGene: Ssc.9364

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