Pig growth hormone,GH ELISA Kit

Instructions
Code CSB-E06813p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth hormone 1
Alternative Names GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
Abbreviation GH1
Uniprot No. P01248
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 2.5 ng/mL-100 ng/mL
Sensitivity 2.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %87-96
1:2Average %95
Range %89-100
1:4Average %94
Range %88-99
1:8Average %95
Range %90-103
Recovery
The recovery of pig GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-102
EDTA plasma (n=4)9892-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
01.905 1.834 1.870
2.51.298 1.282 1.290
7.51.024 1.004 1.014
230.739 0.722 0.731
500.567 0.531 0.549
1000.342 0.338 0.340
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

STRING: 9823.ENSSSCP00000018314

UniGene: Ssc.10492

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