Product Details

Target Name

angiopoietin 1

Alternative Names

Angpt1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit

Abbreviation

ANGPT1

Uniprot No.

O35460

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25 ng/mL-400 ng/mL

Sensitivity

1.56 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	93-109
1:2	Average %	112
	Range %	103-116
1:4	Average %	94
	Range %	84-98
1:8	Average %	90
	Range %	83-94

Recovery

The recovery of rat ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-94
EDTA plasma (n=4)	103	92-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.985	2.845	2.915	2.781
200	2.681	2.579	2.630	2.496
100	2.297	2.213	2.255	2.121
50	1.684	1.622	1.653	1.519
25	1.001	0.956	0.979	0.845
12.5	0.449	0.441	0.445	0.311
6.25	0.251	0.242	0.247	0.113
0	0.132	0.136	0.134

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat ANGPT1 ELISA Kit was designed for the quantitative measurement of Rat ANGPT1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

Target Background

Function
(From Uniprot)

Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.

Gene References into Functions

Overexpression of Ang-1 enhanced the tube formation, migration, and proliferation ability of endothelial progenitor cells. EPC transplantation facilitated occlusion of carotid aneurysms. PMID: 29197611
The study showed for the first time that ANG-1 plays a vital role in the early rather than late phase of adjuvant-induced arthritis pathogenesis making it an attractive non-invasive tool for early diagnosis and prediction of disease prognosis PMID: 24664592
Ang-1-EPC improved cell survival during inflammation. PMID: 24606182
Ang1 was implicated in ASC-induced suppression of neointimal formation. PMID: 23486192
Endothelin-1 regulates ANG-1 production and vascular endothelial growth factor (VEGF)A in proliferating astrocytes. PMID: 22927341
responsible for the vascular hyperreactivity at early hemorrhagic shock through the Tie2-Akt-eNOS pathway PMID: 22869617
The expression of Ang-1 mRNA and TSP-1 in renal tissue of rats after subtotal nephrectomy was significantly up-regulated. PMID: 19821086
Angiopoietin-1 preconditioning enhances survival and functional recovery of mesenchymal stem cell transplantation PMID: 22843181
Prolonged exposure of high concentration of oxygen may cause impairment of lung vascular development by inhibiting expression of Ang-1 in neonatal rats. PMID: 22336306
The change of Ang-1, which is early upregulation and late downregulation, exists in diabetic renal tissue. PMID: 17294737
Wenyang Huoxue Recipe can up-regulate the expression of Ang-l mRNA rats with chronic aristolochic acid nephropathy. PMID: 18471417
Expression of angiopoietin-1 and the angiopoietin receptor dropped markedly after stroke in SHR stroke-prone rats, but was not different after ischemia in SHR and WKY rat strains PMID: 11822892
ang 1 stabilizes testicular microvessels under basal conditions and that a shift in this balance caused by increased ang 2, together with increased VEGF-A, allows vascular leakage, high endothelial cell proliferation PMID: 12773423
Rats engineered to express angiopoietin 1 (Ang-1) constitutively in the lung develop severe pulmonary hypertension. PMID: 14512515
Overexpression of angiopoietin-1 using an adeno-associated virus vector causes pulmonary hypertension in rats. These data provide a novel physiologic animal model for pulmonary hypertension. PMID: 14759414
E2 increases Ang-1 and enhances capillary density in brain under basal conditions through estrogen receptor-alpha, priming the middle cerebral artery territory for survival after experimental focal ischemia PMID: 15637314
Ang-1 promotes neurite outgrowth from dorsal root ganglia cells positive for Tie2. PMID: 15714275
It is concluded that adipocyte-secreted Ang-1 supports neurite outgrowth, which is involved in synaptogenesis. The novel function of Ang-1 appears to play a physiological role in WAT plasticity. PMID: 16493688
Ang1 protects cardiomyocytes from oxidative stress-induced apoptosis by regulating the activity of Proto-Oncogene Proteins c-akt PMID: 17559808
ANGPT1 is necessary for follicular development induced by gonadotropins. PMID: 17989359
Expression of angiopoietin-1 significantly decreased in the pulmonary arteries in chronic hypoxic pulmonary hypertension. PMID: 18073453
Abnormal expression and reciprocal egulation of Angpt1 may play important roles in the development of chronic allograft nephropathy in rat renal allografts. PMID: 18929864
Ang1 modifies basal microvessel permeability coefficients, in keeping with previous reports demonstrating reduced solute flux in inflamed vessels. PMID: 19297368
Ang1 induces neurite outgrowth in PC12 cells in a Tie2-independent, beta1-integrin-FAK-PI3K-Akt-dependent manner. NGF and Ang1 mediate neurite outgrowth via two independent signaling mechanisms. PMID: 19379779
Vascular endothelial growth factor and angiopoietin-1 are involved in liver regeneration following partial hepatectomy. PMID: 11549276

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Subcellular Location

Secreted.

Database Links

KEGG: rno:89807

STRING: 10116.ENSRNOP00000007979

UniGene: Rn.161953

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Rat Angiopoietin 1,ANG-1 ELISA Kit

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