Rat Apolipoprotein A5(apo-A5)ELISA Kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

apolipoprotein A-V

Alternative Names

Apoa5 ELISA kit; Rap3Apolipoprotein A-V ELISA kit; Apo-AV ELISA kit; ApoA-V ELISA kit; Apolipoprotein A5 ELISA kit; Regeneration-associated protein 3 ELISA kit

Abbreviation

APOA5

Uniprot No.

Q9QUH3

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.2 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat apo-A5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	84-90
1:200	Average %	99
	Range %	94-104
1:400	Average %	87
	Range %	84-90
1:800	Average %	100
	Range %	95-105

Recovery

The recovery of rat apo-A5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-95
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.945	1.987	1.966	1.871
10	1.503	1.568	1.536	1.441
5	1.115	1.042	1.079	0.984
2.5	0.717	0.737	0.727	0.632
1.25	0.406	0.415	0.411	0.316
0.625	0.268	0.271	0.270	0.175
0.312	0.161	0.163	0.162	0.067
0	0.096	0.094	0.095

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat APOA5 ELISA Kit was designed for the quantitative measurement of Rat APOA5 protein in serum, plasma, tissue homogenates_x005f_x000D_ . It is a Competitive ELISA kit, its detection range is 31.2 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Target Background

Function
(From Uniprot)

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin.

Gene References into Functions

Our data support the hypothesis that apoA5 promotes hepatic TG storage and therefore contributes to the pathogenesis of non-alcoholic fatty liver disease PMID: 25938357
Demonstrate that apoA-V is secreted into bile, introducing a potential route of delivery of hepatic apoA-V to the gut lumen. PMID: 26505974
The increase in apolipoprotein A mRNA induced by rosiglitazone is not directly mediated by peroxisome proliferator-activated receptor gamma.[apolipoprotein av] PMID: 16570166
apoA-V inefficiently traffics within the secretory pathway, that its intracellular itinerary can be regulated by changes in cellular TG accumulation, and that apoA-V synthesis can modulate VLDL TG mobilization and secretion. PMID: 21115968
decreased apolipoprotein A5 is implicated in insulin resistance-related hypertriglyceridemia in obesity PMID: 20047745
ApoA-V ability to enhance insulin secretion in beta-cells and provide evidence of an internalization pathway involving the midkine as partner. PMID: 19910685
the hypotriglyceridemic gene APOA5 is regulated by thyroid hormone PMID: 15941710
The absence of significant changes in Apoa5 gene expression during chronic fructose-induced triglyceride elevation excludes its major role in mechanisms compensating severe hypertriglyceridemia PMID: 16238453
data suggest that the C terminus of apoA-V is essential for lipid droplet association in transfected hepatoma cells and lipoprotein association in conditioned medium while the signal peptide is required for extracellular trafficking of this protein. PMID: 18450648

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Subcellular Location

Secreted. Early endosome. Late endosome. Golgi apparatus, trans-Golgi network.

Protein Families

Apolipoprotein A1/A4/E family

Tissue Specificity

Liver.

Database Links

KEGG: rno:140638

STRING: 10116.ENSRNOP00000024918

UniGene: Rn.48763

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Code	CSB-E16334r
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.