Rat Bone morphogenetic protein 4,BMP-4 ELISA Kit

Instructions
Code CSB-E04511r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name bone morphogenetic protein 4
Alternative Names Bmp4 ELISA Kit; Bmp-4 ELISA Kit; Dvr-4Bone morphogenetic protein 4 ELISA Kit; BMP-4 ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; BMP-2B ELISA Kit
Abbreviation BMP4
Uniprot No. Q06826
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 94-105  
1:2 Average % 91  
Range % 85-97  
1:4 Average % 86  
Range % 82-90  
1:8 Average % 94  
Range % 90-100  
Recovery
The recovery of rat BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 92 87-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.514 2.651 2.583 2.468  
250 1.867 1.797 1.832 1.717  
125 1.279 1.315 1.297 1.182  
62.5 0.885 0.842 0.864 0.749  
31.2 0.513 0.538 0.526 0.411  
15.6 0.294 0.289 0.292 0.177  
7.8 0.201 0.213 0.207 0.092  
0 0.112 0.118 0.115    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

STRING: 10116.ENSRNOP00000012957

UniGene: Rn.10318

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