Rat C-C chemokine receptor type 2(CCR2) ELISA kit

Instructions
Code CSB-EL004841RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) receptor 2
Alternative Names Ccr2 ELISA Kit; Cmkbr2 ELISA Kit; C-C chemokine receptor type 2 ELISA Kit; C-C CKR-2 ELISA Kit; CC-CKR-2 ELISA Kit; CCR-2 ELISA Kit; CCR2 ELISA Kit; CD antigen CD192 ELISA Kit
Abbreviation CCR2
Uniprot No. O55193
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CCR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 102  
Range % 96-108  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 98  
Range % 92-102  
Recovery
The recovery of rat CCR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-99  
EDTA plasma (n=4) 92 88-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.916 2.815 2.866 2.745  
500 2.232 2.131 2.182 2.061  
250 1.461 1.440 1.451 1.330  
125 0.793 0.802 0.798 0.677  
62.5 0.448 0.437 0.443 0.322  
31.25 0.253 0.242 0.248 0.127  
15.6 0.172 0.169 0.171 0.050  
0 0.120 0.122 0.121    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Receptor for the CCL2, CCL7 and CCL13 chemokines. Receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Transduces a signal by increasing intracellular calcium ion levels (By similarity). Upon CCL2 ligation, mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity).
Gene References into Functions
  1. CCR2 activation in nociceptive dorsal root ganglia neurons plays a role in the pathogenesis of gastric hyperalgesia associated with diabetic gastropathy. PMID: 29359616
  2. Light exposure led to apoptosis of photoreceptors, which expressed CCL2, accelerating an inflammation-mediated cascade by activating and attracting microglia and monocytes and promoting their secretion of CCL2 in the injured position. PMID: 29142497
  3. Expressions of CCR2 mRNA began to increase on the 1st day and reached to the peak on the 7th day, but then started to decrease gradually until 28th day. PMID: 26062549
  4. Results suggest that CCL2/CCR2 in the dorsal root ganglion and spinal cord is involved in the maintenance of lumbar disc herniation-induced pain PMID: 24462503
  5. investigated the expression changes and cellular localization of spinal CCR2 in a rat model of bone cancer induced by Walker 256 cell inoculation. expression of CCR2 in the spinal cord was significantly increased on day 6, 12, and 18 in bone cancer rats PMID: 23511129
  6. Report functional roles for CCL2/CCR2 signaling at the level of the urinary bladder in reducing voiding frequency and somatic sensitivity following CYP-induced cystitis. PMID: 23594826
  7. Overexpression of CCR2 in retinal microglia promotes their chemotaxis in response to chemokines. PMID: 23288990
  8. CCR2 is involved in the process of mast cell degranulation in bladder tissues. PMID: 23049171
  9. The IL-1beta and CCL2-CCR2 signaling cascades play a role in neuron-glia-cytokine interactions and the descending facilitation of neuropathic pain. PMID: 22466130
  10. the important role of CCR2 in neuropathic pain PMID: 21143971
  11. The expressions of CCR2 mRNA and protein increase significantly in cerebral tissue of newborn rat with hypoxic-ischemic brain damage. PMID: 18095591
  12. The data show that CCR2 and CCL2 regulation in the hippocampus changes after pilocarpine-induced status epilepticus or seizures. These changes might be involved in detrimental neuroplasticity and neuroinflammatory changes that occur following seizures. PMID: 20034406
  13. expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and its receptor in the testis of rats undergoing autoimmune orchitis PMID: 14638441
  14. CCR1, CCR2, and CCR5 mRNA expression and tyrosine phosphorylation increased with peak inflammation in adjuvant induced arthritis PMID: 14674010
  15. Cultured neural progenitor cells from the subventricular zone of adult rat brain express chemokine receptor CCR2. PMID: 14732474
  16. Arterial wall and VSMC MCP-1/CCR2 increase with aging. MCP-1 enhances VSMC migration and invasion, and thus, MCP-1/CCR2 signaling may play a role in age-associated arterial remodeling. PMID: 15178559
  17. IFNG priming enhances migration to inflammatory chemokines; IFN-gamma-inducible gene expression is attenuated by high levels of Stat1; physiological regulation of the relative abundance of Stat1 and Stat3 impacts on gene expression PMID: 16148108
  18. MCP-1/CCR2 signaling is directly involved with a chronic compression injury and may contribute to associated neuronal hyperexcitability and neuropathic pain. PMID: 16174730
  19. Data show that the preferential chemokine receptor (CCR)2 ligand, MCP-1/CCL2, elicits calcium transients in primary cultured neurons from various rat brain regions expressing CCR2. PMID: 16196033
  20. CCR2 mediates recruitment of both infiltrating macrophages and resident microglia to specific sites of central nervous system inflammation. PMID: 17484785
  21. These data suggest CCL2 and CCR2 as potential candidates for integrating inflammation and central neuropathic pain after spinal cord injury PMID: 18338959
  22. CCR2 was also expressed in laser-induced CNV but did not increase after PDT. PMID: 18421085
  23. Ccr2 is upregulated by injury to sensory neurons in the lumbar dorsal root ganglia and MCP-1/Ccr2 signaling contributes to subsequent changes in neural activity. These changes may contribute to neuropathic pain. PMID: 16174730

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Expressed in lung, spleen, kidney, thymus and macrophages.
Database Links

KEGG: rno:60463

UniGene: Rn.211983

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