Rat C-C chemokine receptor type 2(CCR2) ELISA kit

Code CSB-EL004841RA
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O55193
Lead Time 7-14 working days
Abbreviation CCR2
Target Name chemokine (C-C motif) receptor 2
Alias CC-CKR-2, CCR2A, CCR2B, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP-1-R, MCP-1 receptor|chemokine (C-C) receptor 2|monocyte chemoattractant protein 1 receptor|monocyte chemotactic protein 1 receptor
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CCR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 102  
Range % 96-108  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 98  
Range % 92-102  
Recovery
The recovery of rat CCR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-99  
EDTA plasma (n=4) 92 88-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.916 2.815 2.866 2.745  
500 2.232 2.131 2.182 2.061  
250 1.461 1.440 1.451 1.330  
125 0.793 0.802 0.798 0.677  
62.5 0.448 0.437 0.443 0.322  
31.25 0.253 0.242 0.248 0.127  
15.6 0.172 0.169 0.171 0.050  
0 0.120 0.122 0.121    
References
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Function Receptor for the CCL2, CCL7 and CCL13 chemokines. Receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Transduces a signal by increasing intracellular calcium ion levels (By similarity). Upon CCL2 ligation, mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity).
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Expressed in lung, spleen, kidney, thymus and macrophages.
Database Links

KEGG: rno:60463

UniGene: Rn.211983

Pathway Chemokine signaling pathway

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