Rat Cardiac Troponin T(cTn-T)ELISA kit

Code CSB-E16443r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat cTn-T ELISA Kit was designed for the quantitative measurement of Rat cTn-T protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL .

Target Name Cardiac Troponin T(cTn-T)
Alternative Names Tnnt2 ELISA Kit; Troponin T ELISA Kit; cardiac muscle ELISA Kit; TnTc ELISA Kit; Cardiac muscle troponin T ELISA Kit; cTnT ELISA Kit
Abbreviation cTn-T
Uniprot No. P50753
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-T in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %82-97
1:2Average %87
Range %81-94
1:4Average %93
Range %87-100
1:8Average %95
Range %89-102
Recovery
The recovery of rat cTn-T spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-105
EDTA plasma (n=4)9588-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
8002.187 2.102 2.145 1.986
4001.731 1.641 1.686 1.527
2001.254 1.204 1.229 1.070
1000.926 0.879 0.903 0.744
500.593 0.554 0.574 0.415
250.302 0.279 0.291 0.132
12.50.241 0.227 0.234 0.075
00.165 0.153 0.159  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. Replacement of the functionally corresponding N-terminal end portion of rat fast skeletal cardiac muscle (RfsTnT) into cardiac muscle troponin T (RcTnT), the observed functional differences associate with a sequence variation. PMID: 23357173
  2. L48Q cTnC reduced crossbridge dependence of thin filament activation in cardiac muscle and changes in Ca(2+) sensitivity of force in response to changes in sarcomere length are at least partially dependent on properties of thin filament troponin. PMID: 22865385
  3. cTnC, cTnI, cTnT and cTm are not only present in myofilaments of ventricular cardiomyocytes in culture but are also within their nuclei; significantly, these four proteins appear between days 3 and 5 in both myofilaments and nuclei PMID: 22364878
  4. Early single cTnT measurement correlates with infarct size in rats, and a cutoff value of 5.1 mug/l provides good sensitivity and specificity to predict congestive heart failure with secondary pulmonary hypertension. PMID: 20537565
  5. The time course of cTnT elevation was temporally associated with evidence of increased lipid peroxidation in the rat heart. PMID: 20711602
  6. a novel role for cTnT as a dual-specificity sarcomeric A-kinase anchoring protein PMID: 21056973
  7. Studies show that the rat Tnnt2-rtTA;TetO-Cre mice transgenic line a valuable genetic tool for analysis of spatiotemporal gene function and cardiomyocyte lineage tracing during developmental and postnatal period. PMID: 20014345
  8. The mu-calpain-mediated proteolytic modification of TnT may act as an acute mechanism to adjust muscle contractility under stress conditions. PMID: 16981728
  9. Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T. PMID: 18397962
  10. Mdetabolic inhibition of cardiomyocytes induces a parallel release of intact Tnnt3 and its degradation products, starting only after onset of irreversible cardiomyocyte damage. PMID: 18721805
  11. Study demonstrate profound energetic alterations in DEL-TNT hearts, supporting the notion that inefficient cellular ATP utilization contributes to the pathogenesis of HCM. PMID: 19189074

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Protein Families Troponin T family
Database Links

KEGG: rno:24837

STRING: 10116.ENSRNOP00000049297

UniGene: Rn.9965

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