Recombinant Rat Troponin T, cardiac muscle(Tnnt2)

Code CSB-YP341903RA
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Source Yeast
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Code CSB-EP341903RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341903RA
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Source Baculovirus
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Code CSB-MP341903RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Tnnt2
Uniprot No. P50753
Alternative Names Tnnt2; Troponin T; cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT
Species Rattus norvegicus (Rat)
Expression Region 2-299
Target Protein Sequence SDAEEEVVE YEEEQEEEDW SEEEEDEQEE AVEEEDGEAE PDPEGEAEAE EDKAEEVGPD EEARDAEDGP VEDSKPKPSR LFMPNLVPPK IPDGERVDFD DIHRKRMEKD LNELQTLIEA HFENRKKEEE ELISLKDRIE KRRAERAEQQ RIRNEREKER QNRLAEERAR REEEENRRKA EDEARKKKAL SNMMHFGGYI QKAQTERKSG KRQTEREKKK KILAERRKVL AIDHLNEDQL REKAKELWQS IHNLEAEKFD LQEKFKQQKY EINVLRNRIN DNQKVSKTRG KAKVTGRWK
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. Replacement of the functionally corresponding N-terminal end portion of rat fast skeletal cardiac muscle (RfsTnT) into cardiac muscle troponin T (RcTnT), the observed functional differences associate with a sequence variation. PMID: 23357173
  2. L48Q cTnC reduced crossbridge dependence of thin filament activation in cardiac muscle and changes in Ca(2+) sensitivity of force in response to changes in sarcomere length are at least partially dependent on properties of thin filament troponin. PMID: 22865385
  3. cTnC, cTnI, cTnT and cTm are not only present in myofilaments of ventricular cardiomyocytes in culture but are also within their nuclei; significantly, these four proteins appear between days 3 and 5 in both myofilaments and nuclei PMID: 22364878
  4. Early single cTnT measurement correlates with infarct size in rats, and a cutoff value of 5.1 mug/l provides good sensitivity and specificity to predict congestive heart failure with secondary pulmonary hypertension. PMID: 20537565
  5. The time course of cTnT elevation was temporally associated with evidence of increased lipid peroxidation in the rat heart. PMID: 20711602
  6. a novel role for cTnT as a dual-specificity sarcomeric A-kinase anchoring protein PMID: 21056973
  7. Studies show that the rat Tnnt2-rtTA;TetO-Cre mice transgenic line a valuable genetic tool for analysis of spatiotemporal gene function and cardiomyocyte lineage tracing during developmental and postnatal period. PMID: 20014345
  8. The mu-calpain-mediated proteolytic modification of TnT may act as an acute mechanism to adjust muscle contractility under stress conditions. PMID: 16981728
  9. Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T. PMID: 18397962
  10. Mdetabolic inhibition of cardiomyocytes induces a parallel release of intact Tnnt3 and its degradation products, starting only after onset of irreversible cardiomyocyte damage. PMID: 18721805
  11. Study demonstrate profound energetic alterations in DEL-TNT hearts, supporting the notion that inefficient cellular ATP utilization contributes to the pathogenesis of HCM. PMID: 19189074

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Protein Families Troponin T family
Database Links

KEGG: rno:24837

STRING: 10116.ENSRNOP00000049297

UniGene: Rn.9965

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