Rat Cathepsin S(CTSS) ELISA kit

Instructions
Code CSB-EL006204RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cathepsin S
Alternative Names Ctss ELISA Kit; Cathepsin S ELISA Kit; EC 3.4.22.27 ELISA Kit
Abbreviation CTSS
Uniprot No. Q02765
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CTSS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-98  
1:2 Average % 97  
Range % 93-105  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 98  
Range % 94-102  
Recovery
The recovery of rat CTSS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 103 98-110  
EDTA plasma (n=4) 84 80-89  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.563 2.457 2.510 2.395  
500 1.997 2.076 2.037 1.922  
250 1.378 1.404 1.391 1.276  
125 0.877 0.821 0.849 0.734  
62.5 0.567 0.554 0.561 0.446  
31.25 0.358 0.331 0.345 0.230  
15.6 0.227 0.240 0.234 0.119  
0 0.113 0.117 0.115    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
Gene References into Functions
  1. Findings demonstrate that neuronal reactive oxygen species promote maturation of microglial CatS which facilitates activation of NMDA in the spinal dorsal horn. PMID: 28039043
  2. Inhibition of cathepsin S release is one mechanism that is responsible for purinergic P2X7 antagonist efficacy in neuropathic pain. PMID: 20629190
  3. Activated rat hepatic stellate cells express the class II transactivator, the invariant chain (CD74), the MHC class II molecules, as well as cathepsin S, all of which are known to be responsible for the initial steps of successful antigen presentation. PMID: 17178165
  4. endogenous CatS released by peripheral macrophages contributes to the maintenance of neuropathic hyperalgesia following nerve injury PMID: 17250968
  5. There is an altered expression of cathepsin S and cathepsin X in the spinal cord of a neuropathic pain model; including cellular distribution and pain development. PMID: 18700000
  6. under neuropathic conditions, noxious stimulation of primary afferent fibers induces release of CatS from microglia, which liberates FKN from dorsal horn neurons, thereby contributing to the amplification and maintenance of chronic pain PMID: 19474321

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Subcellular Location Lysosome. Secreted. Cytoplasmic vesicle, phagosome.
Protein Families Peptidase C1 family
Tissue Specificity Highest levels occur in the ileum followed by spleen, brain, thyroid, ovary and uterus. Low levels are found in the liver, kidney, jejunum and lung with lowest levels in the heart.
Database Links

KEGG: rno:50654

UniGene: Rn.11347

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