Recombinant Rat Cathepsin S (Ctss)

Code CSB-YP006204RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP006204RA
MSDS
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Source E.coli
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Code CSB-EP006204RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006204RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP006204RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ctss
Uniprot No.
Alternative Names
Ctss; Cathepsin S; EC 3.4.22.27
Species
Rattus norvegicus (Rat)
Expression Region
113-330
Target Protein Sequence
LPDSVDWR EKGCVTNVKY QGSCGSCWAF SAEGALEGQL KLKTGKLVSL SAQNLVDCST EEKYGNKGCG GGFMTEAFQY IIDTSIDSEA SYPYKAMDEK CLYDPKNRAA TCSRYIELPF GDEEALKEAV ATKGPVSVGI DDASHSSFFL YQSGVYDDPS CTENMNHGVL VVGYGTLDGK DYWLVKNSWG LHFGDQGYIR MARNNKNHCG IASYCSYPEI
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
Gene References into Functions
  1. Findings demonstrate that neuronal reactive oxygen species promote maturation of microglial CatS which facilitates activation of NMDA in the spinal dorsal horn. PMID: 28039043
  2. Inhibition of cathepsin S release is one mechanism that is responsible for purinergic P2X7 antagonist efficacy in neuropathic pain. PMID: 20629190
  3. Activated rat hepatic stellate cells express the class II transactivator, the invariant chain (CD74), the MHC class II molecules, as well as cathepsin S, all of which are known to be responsible for the initial steps of successful antigen presentation. PMID: 17178165
  4. endogenous CatS released by peripheral macrophages contributes to the maintenance of neuropathic hyperalgesia following nerve injury PMID: 17250968
  5. There is an altered expression of cathepsin S and cathepsin X in the spinal cord of a neuropathic pain model; including cellular distribution and pain development. PMID: 18700000
  6. under neuropathic conditions, noxious stimulation of primary afferent fibers induces release of CatS from microglia, which liberates FKN from dorsal horn neurons, thereby contributing to the amplification and maintenance of chronic pain PMID: 19474321

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Subcellular Location
Lysosome. Secreted. Cytoplasmic vesicle, phagosome.
Protein Families
Peptidase C1 family
Tissue Specificity
Highest levels occur in the ileum followed by spleen, brain, thyroid, ovary and uterus. Low levels are found in the liver, kidney, jejunum and lung with lowest levels in the heart.
Database Links

KEGG: rno:50654

UniGene: Rn.11347

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