Rat Interferon α,IFN-α ELISA Kit

Rat Interferon α,IFN-α ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

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Customer Reviews and Q&A

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Rat Interferon α,IFN-α ELISA Kit

Citations (2) Protocol MSDS

Code	CSB-E08637r
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (2)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

Interferon α,IFN-α

Alternative Names

Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit

Abbreviation

IFNA1

Uniprot No.

P05011

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-91
1:2	Average %	92
	Range %	85-97
1:4	Average %	99
	Range %	94-104
1:8	Average %	103
	Range %	99-108

Recovery

The recovery of rat IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	96	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.087	2.099	2.093	1.916
1000	1.595	1.574	1.585	1.408
500	1.087	1.096	1.092	0.915
250	0.672	0.695	0.684	0.507
125	0.491	0.457	0.474	0.297
62.5	0.376	0.351	0.364	0.187
31.25	0.287	0.299	0.293	0.116
0	0.175	0.178	0.177

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat IFNA1 ELISA Kit was designed for the quantitative measurement of Rat IFNA1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Sini-san improves duodenal tightJunction integrity in a rat model of functional dyspepsia Chang X.et al,BMC Complement Altern Med.,2017
Investigation of the Therapeutic Efficacy of Codelivery of psiRNA-vascular Endothelial Growth Factor and pIL-4 into Chitosan Nanoparticles in the Breast Tumor Salva E et al,J Pharm Sci.,2013

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■ Q&A

Does this ELISA detect IFNalpha 1 or 2?

Thanks for your inquiry. It is designed for IFN alpha 1.

Function
(From Uniprot)

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

Subcellular Location

Secreted.

Protein Families

Alpha/beta interferon family

Database Links

KEGG: rno:298210

STRING: 10116.ENSRNOP00000051043

UniGene: Rn.196548

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

Jak-STAT signaling pathway PI3K-Akt signaling pathway Necroptosis Natural killer cell mediated cytotoxicity NOD-like receptor signaling pathway Toll-like receptor signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.