Rat Interleukin 5,IL-5 ELISA KIT

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Rat Interleukin 5,IL-5 ELISA KIT

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Protocol

Code	CSB-E07435r-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
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Target Background

Target Name

interleukin 5 (colony-stimulating factor, eosinophil)

Alternative Names

Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit

Abbreviation

IL5

Uniprot No.

Q08125

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

3.12pg/mL-200pg/mL

Sensitivity

1.56pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-96
1:2	Average %	93
	Range %	86-100
1:4	Average %	98
	Range %	91-105
1:8	Average %	94
	Range %	86-100

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
200	2.284	2.364	2.324	2.135
100	1.288	1.360	1.324	1.135
50	0.770	0.818	0.794	0.605
25	0.597	0.559	0.578	0.389
12.5	0.376	0.361	0.368	0.179
6.25	0.306	0.293	0.300	0.111
3.12	0.219	0.208	0.214	0.024
0	0.187	0.191	0.189

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Subcellular Location

Secreted.

Protein Families

IL-5 family

Database Links

STRING: 10116.ENSRNOP00000010729

UniGene: Rn.44227

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Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

Jak-STAT signaling pathway Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway T cell receptor signaling pathway Th1 and Th2 cell differentiation Cell markers

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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