Rat Interleukin 5,IL-5 ELISA KIT

Code CSB-E07435r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 5 (colony-stimulating factor, eosinophil)
Alternative Names Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
Abbreviation IL5
Uniprot No. Q08125
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 82-91  
1:2 Average % 92  
Range % 83-99  
1:4 Average % 101  
Range % 93-107  
1:8 Average % 96  
Range % 90-104  
The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 85-94  
EDTA plasma (n=4) 94 90-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.407 2.457 2.432 2.268  
500 1.768 1.736 1.752 1.588  
250 1.065 1.098 1.082 0.918  
125 0.704 0.735 0.720 0.556  
62.5 0.448 0.469 0.459 0.295  
31.2 0.323 0.300 0.312 0.148  
15.6 0.221 0.229 0.225 0.061  
0 0.161 0.167 0.164    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Subcellular Location Secreted
Protein Families IL-5 family
Database Links

STRING: 10116.ENSRNOP00000010729

UniGene: Rn.44227


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