Rat Myoglobin,MYO/MB ELISA Kit

Code CSB-E08049r
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
myoglobin
Alternative Names
Mb ELISA Kit; Myoglobin ELISA Kit
Abbreviation
MB
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
3.9 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MYO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-100
1:2Average %92
Range %87-100
1:4Average %97
Range %90-105
1:8Average %87
Range %83-96
Recovery
The recovery of rat MYO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9688-99
EDTA plasma (n=4)9591-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
10002.064 2.069 2.067 1.922
5001.743 1.754 1.749 1.604
2501.355 1.363 1.359 1.214
1250.954 0.962 0.958 0.813
62.50.718 0.728 0.723 0.578
31.250.498 0.502 0.500 0.355
15.60.337 0.343 0.340 0.195
00.143 0.146 0.145  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat Myoglobin(MB) ELISA Kit was designed for the quantitative measurement of Rat Myoglobin(MB) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .

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Target Background

Function
(From Uniprot)
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Gene References into Functions
  1. In rhabdomyolytic rats, the level of heme and free iron in cytoplasm and mitochondria of kidney cells is remarkably increased while inhibition of proteolysis results in further elevation of myoglobin content in the renal tissue. PMID: 27329933
  2. After reperfusion, there was no significant increase in dialysate myoglobin concentration. MDL-28170 did not affect dialysate myoglobin concentration during occlusion or after reperfusion. PMID: 24256333
  3. Amphetamine-induced serum myoglobin release is a potential biomarker and possibly a factor in AMPH-induced toxicity processes. PMID: 23608161
  4. bradykinin-induced NO does not interact significantly with cellular Mb to produce an NMR-detectable quantity of metMb in the perfused rat myocardium PMID: 14766675
  5. determination of myoglobin concentration and succinate dehydrogenase activity in serial sections indicate that myoglobin can lead to a substantial reduction (18-60%) of the extracellular oxygen tension required to prevent an anoxic core in muscle cells PMID: 15048578
  6. Mb-facilitated diffusion has a higher poise in skeletal muscle than in myocardium. PMID: 17038435
  7. Moreover, the similar Q10 for the DMb of solution versus cell Mb suggests that any temperature-dependent alteration of the postulated cell matrix does not significantly affect protein mobility. PMID: 17218454
  8. The nitrite reductase activity of deoxymyoglobin reduces nitrite approximately 36 times faster than deoxyhemoglobin because of its lower heme redox potential. PMID: 17293481
  9. intermittent hypobaric hypoxia decreased myocardial citrate synthase activity and myoglobin and decreased myocardial lactate dehydrogenase activity PMID: 19308875
  10. Data suggest that rhabdomyolysis-induced kidney damage involves direct interaction of myoglobin with mitochondria possibly resulting in iron ions release from myoglobin's heme, which promotes the peroxidation of mitochondrial membranes. PMID: 19545623

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Globin family
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