Recombinant Rat Myoglobin (Mb)

Code CSB-YP013529RA
MSDS
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Source Yeast
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Code CSB-EP013529RA
MSDS
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Source E.coli
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Code CSB-EP013529RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013529RA
MSDS
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Source Baculovirus
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Code CSB-MP013529RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mb
Uniprot No.
Alternative Names
Mb; Myoglobin
Species
Rattus norvegicus (Rat)
Expression Region
1-154
Target Protein Sequence
MGLSDGEWQM VLNIWGKVEG DLAGHGQEVL ISLFKAHPET LEKFDKFKNL KSEEEMKSSE DLKKHGCTVL TALGTILKKK GQHAAEIQPL AQSHATKHKI PVKYLEFISE VIIQVLKKRY SGDFGADAQG AMSKALELFR NDIAAKYKEL GFQG
Protein Length
Full Length Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Gene References into Functions
  1. In rhabdomyolytic rats, the level of heme and free iron in cytoplasm and mitochondria of kidney cells is remarkably increased while inhibition of proteolysis results in further elevation of myoglobin content in the renal tissue. PMID: 27329933
  2. After reperfusion, there was no significant increase in dialysate myoglobin concentration. MDL-28170 did not affect dialysate myoglobin concentration during occlusion or after reperfusion. PMID: 24256333
  3. Amphetamine-induced serum myoglobin release is a potential biomarker and possibly a factor in AMPH-induced toxicity processes. PMID: 23608161
  4. bradykinin-induced NO does not interact significantly with cellular Mb to produce an NMR-detectable quantity of metMb in the perfused rat myocardium PMID: 14766675
  5. determination of myoglobin concentration and succinate dehydrogenase activity in serial sections indicate that myoglobin can lead to a substantial reduction (18-60%) of the extracellular oxygen tension required to prevent an anoxic core in muscle cells PMID: 15048578
  6. Mb-facilitated diffusion has a higher poise in skeletal muscle than in myocardium. PMID: 17038435
  7. Moreover, the similar Q10 for the DMb of solution versus cell Mb suggests that any temperature-dependent alteration of the postulated cell matrix does not significantly affect protein mobility. PMID: 17218454
  8. The nitrite reductase activity of deoxymyoglobin reduces nitrite approximately 36 times faster than deoxyhemoglobin because of its lower heme redox potential. PMID: 17293481
  9. intermittent hypobaric hypoxia decreased myocardial citrate synthase activity and myoglobin and decreased myocardial lactate dehydrogenase activity PMID: 19308875
  10. Data suggest that rhabdomyolysis-induced kidney damage involves direct interaction of myoglobin with mitochondria possibly resulting in iron ions release from myoglobin's heme, which promotes the peroxidation of mitochondrial membranes. PMID: 19545623

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Protein Families
Globin family
Database Links
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