Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit

Code CSB-E08418r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat PI3K ELISA Kit was designed for the quantitative measurement of Rat PI3K protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name Phosphotylinosital 3 kinase,PI3K
Abbreviation PI3K
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %89-95
1:2Average %103
Range %99-107
1:4Average %93
Range %88-94
1:8Average %99
Range %95-103
Recovery
The recovery of rat PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9691-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.860 2.778 2.819 2.679
5002.231 2.222 2.227 2.087
2501.379 1.404 1.392 1.252
1250.748 0.761 0.755 0.615
62.50.464 0.448 0.456 0.316
31.20.307 0.302 0.305 0.165
15.60.218 0.211 0.215 0.075
00.141 0.139 0.140  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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