Rat Platelet glycoprotein 4(CD36) ELISA kit

Code CSB-EL004927RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat CD36 ELISA Kit was designed for the quantitative measurement of Rat CD36 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name CD36 molecule (thrombospondin receptor)
Alternative Names Cd36 ELISA Kit; FatPlatelet glycoprotein 4 ELISA Kit; Adipocyte membrane protein ELISA Kit; Fatty acid translocase ELISA Kit; Fatty acid transport protein ELISA Kit; Glycoprotein IIIb ELISA Kit; GPIIIB ELISA Kit; PAS IV ELISA Kit; PAS-4 ELISA Kit; Platelet glycoprotein IV ELISA Kit; GPIV ELISA Kit; CD antigen CD36 ELISA Kit
Abbreviation CD36
Uniprot No. Q07969
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CD36 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 94-101  
1:2 Average % 98  
Range % 91-102  
1:4 Average % 93  
Range % 84-97  
1:8 Average % 92  
Range % 84-96  
Recovery
The recovery of rat CD36 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 105 95-110  
EDTA plasma (n=4) 99 90-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.504 2.453 2.479 2.305  
10 2.040 1.922 1.981 1.807  
5 1.285 1.313 1.299 1.125  
2.5 0.733 0.745 0.739 0.565  
1.25 0.430 0.456 0.443 0.269  
0.625 0.326 0.342 0.334 0.160  
0.312 0.245 0.251 0.248 0.074  
0 0.172 0.176 0.174    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine. Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption. In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway. Involved in oral fat perception and preferences. Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions. In taste receptor cells, mediates the induction of an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract. Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis. Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects. As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome. Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway.
Subcellular Location Cell membrane; Multi-pass membrane protein. Membrane raft. Golgi apparatus. Apical cell membrane.
Protein Families CD36 family
Tissue Specificity Expressed at high levels in heart, intestine, spleen, adipose tissue, skeletal muscle and, at lower levels, in testes.
Database Links

STRING: 10116.ENSRNOP00000058398

UniGene: Rn.102418

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