Rat Thrombopoietin,TPO ELISA kit

Instructions
Code CSB-E07378r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name thrombopoietin
Alternative Names Thpo ELISA Kit; Thrombopoietin ELISA Kit
Abbreviation THPO
Uniprot No. P49745
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %86-95
1:2Average %102
Range %97-105
1:4Average %91
Range %85-97
1:8Average %97
Range %91-103
Recovery
The recovery of rat TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.424 2.627 2.526 2.370
101.825 1.724 1.775 1.619
51.140 1.222 1.181 1.025
2.50.765 0.754 0.760 0.604
1.250.481 0.482 0.482 0.326
0.6250.347 0.355 0.351 0.195
0.3120.238 0.229 0.234 0.078
00.155 0.156 0.156
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Subcellular Location Secreted
Protein Families EPO/TPO family
Database Links

KEGG: rno:108348162

STRING: 10116.ENSRNOP00000067269

UniGene: Rn.10576

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